Publication
Exploring the biosurfactant potential of Actinobacteria isolated from Ruta graveolens
| dc.contributor.author | Ferreira, Sílvia | |
| dc.contributor.author | Ribeiro, Inês | |
| dc.contributor.author | Oliveira, Rui S. | |
| dc.contributor.author | Carvalho, Maria de Fátima | |
| dc.date.accessioned | 2024-07-11T13:48:09Z | |
| dc.date.available | 2024-07-11T13:48:09Z | |
| dc.date.issued | 2024-05 | |
| dc.description.abstract | Biosurfactants are surface-active compounds known for their emulsifying properties and numerous advantages, including low environmental toxicity, eco-friendliness, biodegradability and acceptability. These molecules are amphiphilic, containing both hydrophilic and hydrophobic ends, allowing them to interact at the aqueous-non-aqueous interface. Bacterial biosurfactants are interesting due to their various fields of applications, including biomedicine, cosmetics, food, pharmaceutics, water treatment and oil recovery. Actinobacteria are an important group of microorganisms with high potential for producing different bioactive metabolites including antimicrobial, anticancer and other pharmaceutical compounds. Medicinal plants,such as Ruta graveolens, are a rich source of bioactive compounds, and the association of actinobacteria endophytes with such plants are an attractive source for bioprospecting for novel compounds with biomedical and industrial applications. The purpose of this study was to explore the biosurfactant activity of actinobacterial strains previously isolated from R. graveolens. Fifteen previously isolated actinobacterial strains were inoculated into 100 mL Erlenmeyer flasks containing 30 mL of Kim’s broth supplemented with 3% filtered olive oil as a hydrophobic carbon source. After two weeks, biosurfactant productionwas analyzed by measuring the emulsification activity. Eight out of the 15 actinobacterial strains showed emulsification activity. All results were compared with a positive control consisting of Triton X100 (1mg/ml), and a negative control consisting in Kim’s broth. Almost all strains that revealed positive activity are affiliated to the actinobacterial species Tsukamurella tyrosinosolvens (7/8), with one strain belonging to the species Microbacterium gisengiterae. Comparative analysis with the positive control (with an emulsification activity of 60%), indicated that 3/8 samples showed high emulsification activity (>40%), 1 showed moderate activity (37%) and 4/8 showed low/moderate activity (20-30%). The subsequent phases of this study will involve analyzing if the potential biosurfactant compounds can reduce the surface tension and if they can represent new molecules. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Ferreira, S., Ribeiro, I., Oliveira, R. S., & Carvalho, M. de F. (2024). Exploring the biosurfactant potential of actinobacteria isolated from Ruta graveolens. III 1H-TOXRUN International Congress 2024 - Book of Abstracts, Scientific Letters, 1 (1), 81. | pt_PT |
| dc.identifier.issn | 2795-5117 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/25780 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | CESPU | pt_PT |
| dc.relation | The authors also acknowledge the Strategic Funding UIDB/04423/2020 and UIDP/04423/2020, through national funds provided by FCT and the European Regional Development Fund (ERDF). | pt_PT |
| dc.relation.publisherversion | https://publicacoes.cespu.pt/index.php/sl/article/view/261/287 | pt_PT |
| dc.subject | Actinobacteria | pt_PT |
| dc.subject | Medicinal plant | pt_PT |
| dc.subject | Ruta graveolens | pt_PT |
| dc.subject | Biosurfactant | pt_PT |
| dc.title | Exploring the biosurfactant potential of Actinobacteria isolated from Ruta graveolens | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Porto | pt_PT |
| oaire.citation.title | III 1H-TOXRUN International Congress 2024 - Book of Abstracts, Scientific Letters | pt_PT |
| oaire.citation.volume | 1 (1) | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |