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Automatic solid-phase extraction by programmable flow injection coupled to chromatographic fluorimetric determination of fluoroquinolones

2018Journal article Open access
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dc.contributor.authorPeixoto, Patricia S.
dc.contributor.authorSilva, Eduarda M. P.
dc.contributor.authorOsório, Marcelo V.
dc.contributor.authorBarreiros, Luisa
dc.contributor.authorLima, José L. F. C.
dc.contributor.authorSegundo, Marcela A.
dc.date.accessioned2019-06-27T12:21:47Z
dc.date.available2019-06-27T12:21:47Z
dc.date.issued2018
dc.description.abstractFluoroquinolones are broad-spectrum bactericidal agents applied for the treatment of human and veterinary diseases. Their common use and their incorrect disposal foster environmental contamination, namely in water resources, increasing the risk of antimicrobial resistance. Hence, a method based on automatic solid-phase extraction coupled to liquid chromatography and fluorimetric detection is proposed for the determination of fluoroquinolones in environmental waters. For the solid-phase extraction procedure, a commercially available molecularly imprinted polymer targeting fluoroquinolones was trapped inside a flow-through extraction column, integrated into a programmable flow injection system using multisyringe flow injection analysis, where all steps concerning sorbent conditioning, sample loading, matrix removal, and analyte elution were performed under computer control. The eluate resulting from the sample preparation was collected and transferred at-line to chromatographic analysis using a reversed-phase monolithic column coupled to a fluorimetric detector, and isocratic elution with methanol-phosphoric acid (pH 3.0; 5.0 mM) (17.5:82.5, v/v) at a flow rate of 3.5 mL min-1. Sample treatment and chromatographic analysis were performed in tandem, with sample throughput limited by the sample treatment step. Calibration curves based on fluorescence intensity vs. analyte mass were obtained in the range of 10 to 1000 pg for norfloxacin, ciprofloxacin, and enrofloxacin with LOD values of 6-19 ng L-1 for a sample volume of 100 mL, and RSD < 11% at 0.7 ¿g L-1. The method was successfully applied to estuarine river water analysis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1039/C8AY00327Kpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14103
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationPT2020 UID/QUI/50006/2013pt_PT
dc.relationPOCI/01/0145/FEDER/007265pt_PT
dc.relationAUTOMATION OF BIOCHEMICAL ASSAYS USING LAB-ON-VALVE AND BEAD INJECTION TECHNIQUES
dc.relation.publisherversionhttps://pubs.rsc.org/en/content/articlelanding/2018/AY/C8AY00327K#!divAbstractpt_PT
dc.subjectProgrammable flow injectionpt_PT
dc.subjectMonolithic columnpt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectFluoroquinolonept_PT
dc.subjectEnvironmental waterpt_PT
dc.subjectMultisyringept_PT
dc.titleAutomatic solid-phase extraction by programmable flow injection coupled to chromatographic fluorimetric determination of fluoroquinolonespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAUTOMATION OF BIOCHEMICAL ASSAYS USING LAB-ON-VALVE AND BEAD INJECTION TECHNIQUES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F89668%2F2012/PT
oaire.citation.endPage2186pt_PT
oaire.citation.issue18pt_PT
oaire.citation.startPage2180pt_PT
oaire.citation.titleAnalytical Methodspt_PT
oaire.citation.volume10pt_PT
person.familyNameBarreiros
person.givenNameLuisa
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id6508205485
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isProjectOfPublicationb191ce5e-496d-4111-b415-7db84a094710
relation.isProjectOfPublication.latestForDiscoveryb191ce5e-496d-4111-b415-7db84a094710

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