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Impact of brominated flame retardants on lipid metabolism: An in vitro approach

2022Journal article Restricted access
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dc.contributor.authorMaia, Maria Luz
dc.contributor.authorSousa, Sara
dc.contributor.authorPestana, Diogo
dc.contributor.authorFaria, Ana
dc.contributor.authorTeixeira, Diana
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorDomingues, Valentina
dc.contributor.authorCalhau, Conceição
dc.date.accessioned2023-01-25T09:53:07Z
dc.date.embargo2035
dc.date.issued2022
dc.description.abstractBrominated flame retardants (BFRs) are chemicals employed to lower the flammability of several objects. These endocrine disruptor chemicals are lipophilic and persistent in the environment. Due to these characteristics some have been restricted or banned by the European Union, and replaced by several new chemicals, the novel BFRs (NBFRs). BFRs are widely detected in human samples, such as adipose tissue and some were linked with altered thyroid hormone levels, liver toxicity, diabetes and metabolic syndrome in humans. However, the disturbance in lipid metabolism caused by BFRs with emphases to NBFRs remains poorly understood. In this study, we used a pre-adipocyte (3T3-L1) cell line and a hepatocyte (HepG2) cell line to investigate the possible lipid metabolism disruption caused by four BFRs: hexabromobenzene (HBB), pentabromotoluene (PBT), 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB) and hexabromocyclododecane (HBCD). For that purpose, proliferation and Oil Red O assays, as well as, medium fatty acids profile evaluation using Gas chromatography and RNA extraction for quantitative RT-PCR assays were performed. We detected a significant reduction in the proliferation of preadipocytes and an increased lipid accumulation during differentiation caused by HBB. This BFR also lead to a significant increased expression of IL-1β and decreased expression of PGC-1α and adiponectin. Nevertheless, PBT, TBB and HBCD show to increase lipid accumulation in hepatocytes. PBT also display a significant increase of PPARγ gene expression. Lipid accumulation in the cells can occur by diverse mechanisms depending on the BFR. These results highlight the importance of endocrine disruptor compounds in obesity etiopathogeny.pt_PT
dc.description.sponsorshipMaria Luz Maia and Sara Sousa are grateful to FCT (Fundação para a Ciência e a Tecnologia) and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for the Ph.D. grants (SFRH/BD/128817/2017 and SFRH/BD/137516/2018 respectively). The work was supported by UIDB/50006/2020, CHRC (UIDB/04923/2020 and UIDP/04923/2020) with funding from FCT/MCTES (Ministério da Ciência, Tecnologia e Ensino Superior) through national funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.envpol.2021.118639pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21844
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationPortuguese shrimps: nutritional added value and contaminants evaluation. Risk-benefit.
dc.relationalterado para: “Biomonitoring of lipophilic endocrine disruptors in women: cancer biology and Vitamin D pathways” Vitamin D and Endocrine Disruptors relationship in Breast Cancer Women
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationComprehensive Health Research Center - Research, Education, Training and Innovation in Clinical research and Public Health
dc.relationComprehensive Health Research Center - Research, Education, Training and Innovation in Clinical research and Public Health
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0269749121022211?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPentabromotoluenept_PT
dc.subjectHexabromobenzenept_PT
dc.subject2-Ethylhexyl-2,3,4,5-tetrabromobenzoatept_PT
dc.subjectHexabromocyclododecanept_PT
dc.subjectLipid metabolismpt_PT
dc.titleImpact of brominated flame retardants on lipid metabolism: An in vitro approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePortuguese shrimps: nutritional added value and contaminants evaluation. Risk-benefit.
oaire.awardTitlealterado para: “Biomonitoring of lipophilic endocrine disruptors in women: cancer biology and Vitamin D pathways” Vitamin D and Endocrine Disruptors relationship in Breast Cancer Women
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleComprehensive Health Research Center - Research, Education, Training and Innovation in Clinical research and Public Health
oaire.awardTitleComprehensive Health Research Center - Research, Education, Training and Innovation in Clinical research and Public Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F128817%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F137516%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04923%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04923%2F2020/PT
oaire.citation.startPage118639pt_PT
oaire.citation.titleEnvironmental Pollutionpt_PT
oaire.citation.volume294pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGalante Maia
person.familyNameSousa
person.familyNameDelerue-Matos
person.familyNameDomingues
person.givenNameMaria da Luz
person.givenNameSara
person.givenNameCristina
person.givenNameValentina Maria Fernandes
person.identifier.ciencia-id161F-62CC-7A37
person.identifier.ciencia-id8513-3537-E319
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.ciencia-id4E16-791D-6664
person.identifier.orcid0000-0003-4383-3822
person.identifier.orcid0000-0002-8291-1160
person.identifier.orcid0000-0002-3924-776X
person.identifier.orcid0000-0003-3472-849X
person.identifier.ridQ-5273-2018
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id56436939000
person.identifier.scopus-author-id57194554670
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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