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Fucoxanthin’s Optimization from Undaria pinnatifida Using Conventional Heat Extraction, Bioactivity Assays and In Silico Studies

2022Journal article Open access
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dc.contributor.authorLourenço-Lopes, Catarina
dc.contributor.authorFraga-Corral, Maria
dc.contributor.authorSoria-Lopez, Anton
dc.contributor.authorNuñes-Estevez, Bernabe
dc.contributor.authorBarral-Martinez, Marta
dc.contributor.authorSilva, Aurora
dc.contributor.authorLi, Ningyang
dc.contributor.authorLiu, Chao
dc.contributor.authorSimal-Gandara, Jesus
dc.contributor.authorPrieto, Miguel A.
dc.date.accessioned2023-01-24T11:07:18Z
dc.date.available2023-01-24T11:07:18Z
dc.date.issued2022
dc.description.abstractBrown macroalgae are a potential source of natural pigments. Among them, Undaria pinnatifida is recognized for its high concentration of fucoxanthin (Fx), which is a pigment with a wide range of bioactivities. In this study, three independent parameters were optimized for conventional heat extraction (CHE) to maximize the recovery of Fx from Undaria pinnatifida. Optimal conditions (temperature = 45 °C, solvent = 70%, and time = 61 min) extracted 5.1 mg Fx/g dw. Later, the bioactivities of the Fx-rich extracts (antioxidant, antimicrobial, and neuroprotective) were assessed using in vitro and in silico approaches. In vitro assays indicated that Fx has a strong antioxidant capacity and even stronger antimicrobial activity against gram-positive bacteria. This data was supported in silico where Fx established a high binding affinity to DR, a Staphylococcus aureus protein, through aa ALA-8, LEU-21, and other alkane interactions. Finally, the in vitro enzymatic inhibition of AChE using Fx, was further supported using docking models that displayed Fx as having a high affinity for aa TYR72 and THR 75; therefore, the Fx extraction behavior explored in this work may reduce the costs associated with energy and solvent consumption. Moreover, this paper demonstrates the efficiency of CHE when recovering high amounts of Fx from Undaria pinnatifida. Furthermore, these findings can be applied in different industries.pt_PT
dc.description.sponsorshipAuthors are grateful to the Bio Based Industries Joint Undertaking (JU) under grant agreement no. 888003 UP4HEALTH Project (H2020-BBI-JTI-2019). The JU receives support from the European Union’s Horizon 2020 research and innovation program and the Bio Based Industries Consortium. The project SYSTEMIC Knowledge Hub on Nutrition and Food Security, has received funding from national research funding parties in Belgium (FWO), France (INRA), Germany (BLE), Italy (MIPAAF), Latvia (IZM), Norway (RCN), Portugal (FCT), and Spain (AEI) as a result of a joint action by JPI HDHL, JPI-OCEANS, and FACCE-JPI that was launched in 2019 under the ERA-NET ERA-HDHL (n_ 696295).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/antiox11071296pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21815
dc.language.isoengpt_PT
dc.publisherMDPIpt_PT
dc.relationH2020-BBI-JTI-2019pt_PT
dc.relationSustainable and cost-effective production process for the upcycling of olive, grape and nut by-products into 4 natural and healthy ingredients for nutraceutical and cosmetic applications
dc.relationERA-NET Biomarkers for Nutrition and Health implementing the JPI HDHL objectives
dc.relation.publisherversionhttps://www.mdpi.com/2076-3921/11/7/1296pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectFucoxanthinpt_PT
dc.subjectConventional heat extractionpt_PT
dc.subjectUndaria pinnatifidapt_PT
dc.subjectKineticspt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectNeuroprotective activitypt_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectin silico studiespt_PT
dc.subjectDockingpt_PT
dc.titleFucoxanthin’s Optimization from Undaria pinnatifida Using Conventional Heat Extraction, Bioactivity Assays and In Silico Studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSustainable and cost-effective production process for the upcycling of olive, grape and nut by-products into 4 natural and healthy ingredients for nutraceutical and cosmetic applications
oaire.awardTitleERA-NET Biomarkers for Nutrition and Health implementing the JPI HDHL objectives
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/888003/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/696295/EU
oaire.citation.issue7pt_PT
oaire.citation.startPage1296pt_PT
oaire.citation.titleAntioxidantspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStreamH2020
oaire.fundingStreamH2020
person.familyNameSoares da Silva
person.givenNameMaria Aurora
person.identifier1418356
person.identifier.ciencia-id4A1F-0531-3943
person.identifier.orcid0000-0002-8066-360X
person.identifier.scopus-author-id57197468074
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication612a165f-4a85-48ad-b7a4-dba8d5687d9b
relation.isAuthorOfPublication.latestForDiscovery612a165f-4a85-48ad-b7a4-dba8d5687d9b
relation.isProjectOfPublication2f8a7f48-af63-487f-a00e-6fda3d2215c4
relation.isProjectOfPublication1568b451-99e3-434a-86fd-f12e6612ade0
relation.isProjectOfPublication.latestForDiscovery1568b451-99e3-434a-86fd-f12e6612ade0

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