Publication
Laccase bioconjugate and multi-walled carbon nanotubes-based biosensor for bisphenol A analysis
| dc.contributor.author | Bravo, Iria | |
| dc.contributor.author | Prata, Mariana | |
| dc.contributor.author | Torrinha, Álvaro | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.contributor.author | Lorenzo, Encarnación | |
| dc.contributor.author | Morais, Simone | |
| dc.date.accessioned | 2023-01-24T14:24:22Z | |
| dc.date.embargo | 2035 | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Bisphenol A (BPA) is an endocrine disruptor compound that has been detected in aquatic ecosystems. In this work, the development of an electrochemical biosensor for BPA determination based on laccase from Trametes versicolor is reported. A bioconjugate was optimized to maximize the biosensor electrocatalytic activity and stability, which for the first time involved the synergistic effect of this specific enzyme (6.8 UmL−1), chitosan (5 mgmL−1) and the ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate in an optimum 5:5:2 (v/v/v) proportion. This bioconjugate was deposited onto a screen-printed carbon electrode previously modified with multi-walled carbon nanotubes (MWCNTs). Nanostructuration with MWCNTs enlarged the electrocatalytic activity and surface area, thus improving the biosensor performance. The BPA electrochemical reaction follows an EC mechanism at the optimum pH value of 5.0. Linearity up to 12 µM, a sensitivity of (6.59 ± 0.04) × 10-2 μAμM−1 and a detection limit of 8.4 ± 0.3 nM were obtained coupled with high reproducibility (relative standard deviations lower than 6%) and stability (87% of the initial response after one month). The developed biosensor was employed to the analysis of BPA in river water displaying appropriate accuracy (94.6–97.9%) and repeatability (3.1 to 6% relative standard deviations) proving its high potential applicability for in situ environmental analysis. | pt_PT |
| dc.description.sponsorship | The authors are grateful for the financial support of project UIDB/50006/2020 funded by FEDER funds through the POCI and by National Funds through FCT - Fundação para a Ciência e Tecnologia (FCT)/MCTES. Authors also acknowledge funding from Spanish Ministerio de Economía, Industria y Competitividad through project CTQ2017-84309-C2-1-R and Comunidad de Madrid, through project S2018/NMT-4349 (TRANSNANOAVANSENS). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1016/j.bioelechem.2021.108033 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.22/21822 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation | CTQ2017-84309-C2-1-R | pt_PT |
| dc.relation | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S1567539421002966?via%3Dihub | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Bisphenol A | pt_PT |
| dc.subject | Laccase | pt_PT |
| dc.subject | Biosensor | pt_PT |
| dc.subject | Ionic liquid bioconjugate | pt_PT |
| dc.subject | Screen-printed carbon electrode | pt_PT |
| dc.subject | Electroanalysis | pt_PT |
| dc.title | Laccase bioconjugate and multi-walled carbon nanotubes-based biosensor for bisphenol A analysis | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT | |
| oaire.citation.startPage | 108033 | pt_PT |
| oaire.citation.title | Bioelectrochemistry | pt_PT |
| oaire.citation.volume | 144 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| person.familyName | Bravo | |
| person.familyName | Torrinha | |
| person.familyName | Delerue-Matos | |
| person.familyName | Morais | |
| person.givenName | Iria | |
| person.givenName | Álvaro | |
| person.givenName | Cristina | |
| person.givenName | Simone | |
| person.identifier | 1495443 | |
| person.identifier | 1598822 | |
| person.identifier.ciencia-id | 2D1F-FFB3-34B8 | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.ciencia-id | B111-BBFB-F200 | |
| person.identifier.orcid | 0000-0001-7927-1804 | |
| person.identifier.orcid | 0000-0002-9829-8559 | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.orcid | 0000-0001-6433-5801 | |
| person.identifier.rid | K-8781-2013 | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 56060519600 | |
| person.identifier.scopus-author-id | 6603741848 | |
| person.identifier.scopus-author-id | 7007053747 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | closedAccess | pt_PT |
| rcaap.type | article | pt_PT |
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