Publication
Nasal colonization by Staphylococcus aureusin Health Sciences students and analysis of risk fac-tors under a One Health perspective
| dc.contributor.author | Gomes-Sampaio, L. M. | |
| dc.contributor.author | Cláudia-Ferreira, A. | |
| dc.contributor.author | Prata, J. C. | |
| dc.contributor.author | Azevedo, R. M. S. | |
| dc.contributor.author | Pacheco, P. | |
| dc.contributor.author | Campos, Carla | |
| dc.contributor.author | Novais, C. | |
| dc.contributor.author | Peixe, L. | |
| dc.contributor.author | Dinis-Oliveira, R. J. | |
| dc.contributor.author | Coelho, C. | |
| dc.contributor.author | Miranda, C. | |
| dc.contributor.author | Quinteira, S. | |
| dc.contributor.author | Freitas, A. R. | |
| dc.date.accessioned | 2024-07-11T13:45:55Z | |
| dc.date.available | 2024-07-11T13:45:55Z | |
| dc.date.issued | 2023-04 | |
| dc.description.abstract | Staphylococcus aureusis the leading bacterial cause of death globally. Nasal carriage of S. aureusincreases the risk of invasive infections, including by methicillin-resistant S. aureus(MRSA) strains, but studies including Portuguese university students (PUS) are scarce. To analyse the prevalence of methicillin-susceptible S. aureus(MSSA) and MRSA among PUS enrolled in different courses/years (1st-4th) at IUCS-CESPU, characterize their antibiotic resistance profiles, and assess the potential risk factors. Swabs collected during March-December 2022 from anterior nares of 156 volunteers (median 22-years) were processed in mannitol-salt agar and, in parallel, enriched in brain-heart broth with NaCl 6.5% further plated onto ChromID® MRSA SMART. Typical colonies were stored for species identification (MALDITOF-MS) and antibiotic susceptibility testing (disk diffusion; EU-CAST/CLSI guidelines). Each student completed a questionnaire comprising demographic/clinical/social parameters. Statistical analysis was conducted in IBM-SPSS Statistics 26 using binary logistic regression applying a backward stepwise (likelihood ratio) method, with α=0.05, selecting variables using Chi-square tests and Mann-Whitney U tests for which p≤0.20, >10 occurrences, not biologicallycorrelated. Prevalence of MSSA and MRSA (cefoxitin screening) were 28.8% and 1.9%, respectively. From the 45 positive samples, 9% were multidrug-resistant, 38% were resistant to penicillin, 40% to erythromycin, 40% to clindamycin (inducible),7% to cefoxitin, 2% to tetracycline, and 2% to rifampicin. Self-reported frequent contact with animals (OR=3.44, CI 95%: 1.10–10.66) were positively associated with S. aureus, while regular sports participation presented a negative association (OR=0.36, CI 95%: 0.17–0.77). Sports participation was not correlated with self-reported excellent health (χ2=0.680, p=0.409). This is one of the first studies assessing MSSA/MRSA rates in PUS after the COVID-19 pandemics imposing higher self-protection/hygienization. While PUS-MSSA rates are similar to that previously observed, PUS-MRSA rates are slightly higher. Additional samples are being processed to explore future trends and other potential One Health factors influencing MSSA/MRSA colonization. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Gomes-Sampaio, L. M., Cláudia-Ferreira, A., Prata, J. C., Azevedo, R. M. S., Pacheco, P., Campos, C., Novais, C., Peixe, L., Dinis-Oliveira, R. J., Coelho, C., Miranda, C., Quinteira, S., & Freitas, A. R. (2023). Nasal colonization by Staphylococcus aureus in Health Sciences students and analysis of risk factors under a One Health perspective. II TOXRUN International Congress 2023 - Book of Abstracts, Scientific Letters, 1, Artigo Sup 1. https://doi.org/10.48797/sl.2023.79 | pt_PT |
| dc.identifier.doi | 10.48797/sl.2023.79 | pt_PT |
| dc.identifier.issn | 2795-5117 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/25779 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | CESPU | pt_PT |
| dc.relation | Research was partly funded by FCT/MCTES (Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) for the national funds received through the projects UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences, project LA/P/0140/2020 of the Associate Laboratory, Institute for Health and Bioeconomy (i4HB) | pt_PT |
| dc.relation.publisherversion | https://publicacoes.cespu.pt/index.php/sl/article/view/79 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Staphylococcus aureus | pt_PT |
| dc.subject | MRSA | pt_PT |
| dc.subject | University students | pt_PT |
| dc.subject | One Health | pt_PT |
| dc.title | Nasal colonization by Staphylococcus aureusin Health Sciences students and analysis of risk fac-tors under a One Health perspective | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Porto | pt_PT |
| oaire.citation.title | II TOXRUN International Congress 2023 - Book of Abstracts Scientific Letters | pt_PT |
| oaire.citation.volume | 1 (1) | pt_PT |
| person.familyName | Campos | |
| person.givenName | Carla | |
| person.identifier.ciencia-id | A71C-B158-BC1F | |
| person.identifier.orcid | 0000-0002-6220-4469 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
| relation.isAuthorOfPublication | 2d983798-af3d-467b-a97e-e5629edf5409 | |
| relation.isAuthorOfPublication.latestForDiscovery | 2d983798-af3d-467b-a97e-e5629edf5409 |