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Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation

2021-07Journal article Restricted access
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dc.contributor.authorPinho, Susana C. M.
dc.contributor.authorFaria, Miguel A.
dc.contributor.authorMelo, Armindo
dc.contributor.authorPinto, Edgar
dc.contributor.authorAlmeida, Agostinho
dc.contributor.authorAlves, Rui
dc.contributor.authorCabrita, Ana R. J.
dc.contributor.authorFonseca, António J. M.
dc.contributor.authorFerreira, Isabel M. P. V. O.
dc.date.accessioned2021-10-15T14:01:37Z
dc.date.available2021-10-15T14:01:37Z
dc.date.issued2021-07
dc.description.abstractThe capacity of skimmed milk to neutralise increased reactive oxygen species (ROS) and to attenuate nitric oxide (NO) production, as well as to present cytoprotective effect at the intestinal level was assessed after in vitro gastro-intestinal digestion. The impact on ROS modulation was evaluated at a non-cytotoxic concentration of hydrogen peroxide (H2O2) in a co-culture of Caco-2 and HT-29 intestinal cells. In parallel, a cytotoxic concentration of H2O2 was used to study the effect of digested milk against induced cell apoptosis. Concerning induced NO production, it was evaluated using the model lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Results showed that digested milk prevented the increase of basal ROS level in the intestinal epithelium and attenuated NO production by LPS-stimulated macrophage cells. In the H2O2-induced cytotoxicity assay, digested milk had no protection against apoptosis, confirmed by the failure in attenuating activated caspase-3/7.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPinho, S. C. M., et al. (2021). "Effect of skimmed milk on intestinal tract: Prevention of increased reactive oxygen species and nitric oxide formation." International Dairy Journal 118: 105046.pt_PT
dc.identifier.doi10.1016/j.idairyj.2021.105046pt_PT
dc.identifier.issn0958-6946
dc.identifier.urihttp://hdl.handle.net/10400.22/18714
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0958694621000741?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectSkimmed milkpt_PT
dc.subjectReactive oxygen speciespt_PT
dc.subjectNitric oxide formationpt_PT
dc.titleEffect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleInternational Dairy Journalpt_PT
oaire.citation.volume118pt_PT
person.familyNamePinto
person.givenNameEdgar
person.identifier.ciencia-id271F-B7DF-8FAB
person.identifier.orcid0000-0002-8021-4783
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationeaf9fc86-1a1c-437f-adee-d28040aa7f2f
relation.isAuthorOfPublication.latestForDiscoveryeaf9fc86-1a1c-437f-adee-d28040aa7f2f

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