Publication
Iron oxide/gold core/shell nanomagnetic probes and CdS biolabels for amplified electrochemical immunosensing of Salmonella typhimurium
| dc.contributor.author | Freitas, Maria | |
| dc.contributor.author | Viswanathan, Subramanian | |
| dc.contributor.author | Nouws, Henri P. A. | |
| dc.contributor.author | Oliveira, M. Beatriz P. P. | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.date.accessioned | 2013-09-26T15:10:39Z | |
| dc.date.available | 2013-09-26T15:10:39Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | There is an imminent need for rapid methods to detect and determine pathogenic bacteria in food products as alternatives to the laborious and time-consuming culture procedures. In this work, an electrochemical immunoassay using iron/gold core/shell nanoparticles (Fe@Au) conjugated with anti-Salmonella antibodies was developed. The chemical synthesis and functionalization of magnetic and gold-coated magnetic nanoparticles is reported. Fe@Au nanoparticles were functionalized with different self-assembled monolayers and characterized using ultraviolet-visible spectrometry, transmission electron microscopy, and voltammetric techniques. The determination of Salmonella typhimurium, on screen-printed carbon electrodes, was performed by square-wave anodic stripping voltammetry through the use of CdS nanocrystals. The calibration curve was established between 1×101 and 1×106 cells/mL and the limit of detection was 13 cells/mL. The developed method showed that it is possible to determine the bacteria in milk at low concentrations and is suitable for the rapid (less than 1 h) and sensitive detection of S. typhimurium in real samples. Therefore, the developed methodology could contribute to the improvement of the quality control of food samples. | por |
| dc.identifier.doi | 10.1016/j.bios.2013.07.048 | pt_PT |
| dc.identifier.issn | 0956-5663 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/2012 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.relation.ispartofseries | Biosensors and Bioelectronics; Vol. 51 | |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0956566313005204 | por |
| dc.subject | Salmonella typhimurium | por |
| dc.subject | Electrochemical immunoassay | por |
| dc.subject | Magnetic nanoparticles | por |
| dc.subject | Cadmium nanocrystals | por |
| dc.subject | Labeled detection | por |
| dc.title | Iron oxide/gold core/shell nanomagnetic probes and CdS biolabels for amplified electrochemical immunosensing of Salmonella typhimurium | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 200 | por |
| oaire.citation.startPage | 195 | por |
| oaire.citation.volume | 51 | por |
| person.familyName | Delerue-Matos | |
| person.givenName | Cristina | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 6603741848 | |
| rcaap.rights | closedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | 09f6a7bd-2f15-42b0-adc5-04bd22210519 | |
| relation.isAuthorOfPublication.latestForDiscovery | 09f6a7bd-2f15-42b0-adc5-04bd22210519 |