Repository logo
 
Publication

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

2019-03Journal article Open access
Simple item page
dc.contributor.authorSilva, Nádia
dc.contributor.authorMagalhães, Júlia M.C.S.
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorOliva-Teles, MT
dc.contributor.authorFreire, Cristina
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2020-07-31T10:27:10Z
dc.date.available2020-07-31T10:27:10Z
dc.date.issued2019-03
dc.description.abstractPolymeric ion selective electrodes are highly sensitive to changes in zero current ion flow and this offers a route to signal amplification in label-free potentiometric immunosensors. In this work, a label-free potentiometric immunosensor toward Salmonella typhimurium (ST) assembled in a home-made pipette-tip electrode is described. The signal-output amplification was implemented on a gold nanoparticle polymer inclusion membrane (AuNPs-PIM) which was used as sensing platform and for antibody immobilization. Additionally, a marker ion was used to detect the antibody-antigen binding event at the electrode surface. The immunosensor construction was performed in several steps: i) gold salt ions extraction in PVC membrane; ii) AuNPs formation using Na2EDTA as reduction agent; iii) antibody anti-Salmonella conjugation on AuNPs-PIM in pipette-tip electrodes. The potential shift observed in potentiometric measurements was derived simply from the blocking effect in the ionic flux caused by antigen-antibody conjugation, without no extra steps, mimetizing the ion-channel sensors. A detection limit of 6 cells mL-1 was attained. As proof-of-concept, recovery studies were performed in spiked commercial apple juice samples with success. Due to the simplicity of use, the appealing cost of equipment and sensor production and being able to provide a quick analytical response (less than 1 h for a complete assay, including sample preparation for analysis), this scheme represents a good prototype device for the detection of foodborne pathogens like ST or other immune-responsive bacteria.pt_PT
dc.description.sponsorshipThis work received financial support from the European Union (FEDER funds through COMPETE) and National Funds from Portugal; FCT-Fundação para a Ciência e a Tecnologia through projects UID/QUI/50006/2013 and Norte-01-0145-FEDER-000011-RL1-QUALIFOOD. N.F.D Silva and M.F. Barroso are gratefully to FCT grant SFRH/BD/112414/2015 and SFRH/BPD/78845/2011, financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by FSE and MCTES.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.talanta.2018.10.024pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16144
dc.language.isoengpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0039914018310609?via%3Dihub#!pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectMaluspt_PT
dc.subjectSalmonella typhimuriumpt_PT
dc.subjectPotentiometrypt_PT
dc.subjectGold nanoparticlespt_PT
dc.subjectPolymer inclusion membranespt_PT
dc.subjectImmunosensorpt_PT
dc.titleIn situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F78845%2F2011/PT
oaire.citation.endPage142pt_PT
oaire.citation.startPage134pt_PT
oaire.citation.titleTalantapt_PT
oaire.citation.volume194pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameSilva
person.familyNamede Sá Barroso
person.familyNameOliva-Teles
person.familyNameDelerue-Matos
person.givenNameNádia
person.givenNameMaria de Fátima
person.givenNameMaria Teresa
person.givenNameCristina
person.identifier.ciencia-id1A1B-4743-7EC5
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-7759-5768
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0002-8577-3461
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridA-2400-2016
person.identifier.ridD-8428-2013
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id56622286500
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id6506976504
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa2838665-cbc7-48cb-954d-9e46c73c3aaa
relation.isAuthorOfPublication3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isAuthorOfPublication04d04f38-6e64-4b82-afd5-5bbc0a56506d
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isProjectOfPublication5c06a623-3499-408c-aef9-0a1f68cb1d02
relation.isProjectOfPublication3bd5dba6-8b15-4deb-9b6a-b383334b593d
relation.isProjectOfPublication.latestForDiscovery5c06a623-3499-408c-aef9-0a1f68cb1d02

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ART_DELERUE_2019_22.pdf
Size:
2.59 MB
Format:
Adobe Portable Document Format
Download

Collections

ISEP – GRAQ – Artigos