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Orientador(es)
Resumo(s)
This project aims to check whether there is an influence on the bacterial growth
due to the presence of the adipocyte’s secretome by creating an in vitro model
modulating obesity. Due to the rise of obesity all over the world, to discover a
link between it and bacterial infections is crucial to better understand exactly what
mechanisms hide behind it. Several different bacterial strains will be used to test
the model. The strains to be used in this project were selected taking in
consideration the most common infections, ranging from enterobacteria, gram
positive and gram negative (Esherichia coli (ATCC 25922), Pseudomonas
aeruginosa (ATCC 10145, Kebsiela pneumoniae ATCC BAA-1705, Salmonella
enterica ATCC 13076, Proteus mirabilis ATCC 25933, Sthapylococcus aureus
ATCC 25923, Sthapylococcus epidermis ATCC 14990 and Mycobacterium
smegmatis ATCC 19420). In order to mimic the adipocyte’s secretome 3T3-L1
pre-adipocytes were selected to be grown and differentiated, which will later be
incubated in serum-free DMEM, by harvesting the medium after a period of 24
hours a medium composed by DMEM and the 3T3-L1 adipocyte’s is obtained.
The bacterial strains will be grown in liquid M9 minimal medium and then placed
in 96 wells microplates. Each strain will be placed within 3 different mediums,
M9, to ensure the bacteria is alive, used as a control, DMEM and CM. With these
last two mediums we will be able to verify the difference in growth caused by the
adipocyte’s secretome. The growth will be monitored through optical density, at
a wavelength of 620nm during an expected period of 2 weeks, which may be
adjusted impending on the results obtained.
Descrição
Palavras-chave
Adipocyte secretome Bacterial infections Hyperglicemic environment