Publication
Effects of storage, processing and proteolytic digestion on microcystin-LR concentration in edible clams
| dc.contributor.author | Freitas, Marisa | |
| dc.contributor.author | Azevedo, Joana | |
| dc.contributor.author | Carvalho, António Paulo | |
| dc.contributor.author | Campos, Alexandre | |
| dc.contributor.author | Vasconcelos, Vítor | |
| dc.date.accessioned | 2015-05-20T15:13:08Z | |
| dc.date.available | 2015-05-20T15:13:08Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Accumulation of microcystin-LR (MC-LR) in edible aquatic organisms, particularly in bivalves, is widely documented. In this study, the effects of food storage and processing conditions on the free MC-LR concentration in clams (Corbicula fluminea) fed MC-LR-producing Microcystisaeruginosa (1 × 105 cell/mL) for four days, and the bioaccessibility of MC-LR after in vitro proteolytic digestion were investigated. The concentration of free MC-LR in clams decreased sequentially over the time with unrefrigerated and refrigerated storage and increased with freezing storage. Overall, cooking for short periods of time resulted in a significantly higher concentration (P < 0.05) of free MC-LR in clams, specifically microwave (MW) radiation treatment for 0.5 (57.5%) and 1 min (59%) and boiling treatment for 5 (163.4%) and 15 min (213.4%). The bioaccessibility of MC-LR after proteolytic digestion was reduced to 83%, potentially because of MC-LR degradation by pancreatic enzymes. Our results suggest that risk assessment based on direct comparison between MC-LR concentrations determined in raw food products and the tolerable daily intake (TDI) value set for the MC-LR might not be representative of true human exposure. | por |
| dc.identifier.doi | 10.1016/j.fct.2014.01.041 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/6066 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Food and Chemical Toxicology | por |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0278691514000556 | por |
| dc.subject | Bioaccessibility | por |
| dc.subject | Clams | por |
| dc.subject | Food processing | por |
| dc.subject | Food storage | por |
| dc.subject | Microcystin-LR | por |
| dc.subject | Proteolytic digestion | por |
| dc.title | Effects of storage, processing and proteolytic digestion on microcystin-LR concentration in edible clams | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 223 | por |
| oaire.citation.startPage | 217 | por |
| person.familyName | Freitas | |
| person.givenName | Marisa | |
| person.identifier.ciencia-id | 131C-AD64-7E5F | |
| person.identifier.orcid | 0000-0003-1886-4942 | |
| person.identifier.scopus-author-id | 7102366695 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | ee4b4693-5911-46e5-9247-129bafa5a864 | |
| relation.isAuthorOfPublication.latestForDiscovery | ee4b4693-5911-46e5-9247-129bafa5a864 |