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An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment

2024-12-19Research article Embargoed access
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dc.contributor.authorAlmeida, Ana C.
dc.contributor.authorAlmeida, Ana C.
dc.contributor.authorRocha, Hélder
dc.contributor.authorRocha, Hélder
dc.contributor.authorRaas, Maximilian W. D.
dc.contributor.authorRaas, Maximilian W. D.
dc.contributor.authorWitte, Hanh
dc.contributor.authorWitte, Hanh
dc.contributor.authorSommer, Ralf J.
dc.contributor.authorSommer, Ralf J.
dc.contributor.authorSnel, Berend
dc.contributor.authorSnel, Berend
dc.contributor.authorKops, Geert J. P. L.
dc.contributor.authorKops, Geert J. P. L.
dc.contributor.authorGassmann, Reto
dc.contributor.authorGassmann, Reto
dc.contributor.authorMaiato, Helder
dc.contributor.authorMaiato, Helder
dc.date.accessioned2025-06-20T08:38:18Z
dc.date.available2025-06-20T08:38:18Z
dc.date.issued2024-12-19
dc.description.abstractChromosome alignment during mitosis can occur as a consequence of bi-orientation or is assisted by the CENP-E (kinesin-7) motor at kinetochores. We previously found that Indian muntjac chromosomes with larger kinetochores bi-orient more efficiently and are biased to align in a CENP-E-independent manner, suggesting that CENP-E dependence for chromosome alignment negatively correlates with kinetochore size. Here, we used targeted phylogenetic profiling of CENP-E in monocentric (localized centromeres) and holocentric (centromeres spanning the entire chromosome length) clades to test this hypothesis at an evolutionary scale. We found that, despite being present in common ancestors, CENP-E was lost more frequently in taxa with holocentric chromosomes, such as Hemiptera and Nematoda. Functional experiments in two nematodes with holocentric chromosomes in which a CENP-E ortholog is absent (Caenorhabditis elegans) or present (Pristionchus pacificus) revealed that targeted expression of human CENP-E to C. elegans kinetochores partially rescued chromosome alignment defects associated with attenuated polar-ejection forces, whereas CENP-E inactivation in P. pacificus had no detrimental effects on mitosis and viability. These data showcase the dispensability of CENP-E for mitotic chromosome alignment in species with larger kinetochores.por
dc.identifier.citationAlmeida, A. C., Rocha, H., Raas, M. W. D., Witte, H., Sommer, R. J., Snel, B., Kops, G. J. P. L., Gassmann, R., & Maiato, H. (2024). An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment. Journal of Cell Science, 137(24), jcs263466. https://doi.org/10.1242/jcs.263466
dc.identifier.doi10.1242/jcs.263466
dc.identifier.eissn1477-9137
dc.identifier.issn0021-9533
dc.identifier.urihttp://hdl.handle.net/10400.22/30167
dc.language.isoeng
dc.peerreviewedyes
dc.publisherThe Company of Biologists
dc.relationPTDC/MED-ONC/3479/2020 and PTDC/BIA-CEL/1321/2021
dc.relation.hasversionhttps://journals.biologists.com/jcs/article/137/24/jcs263466/364812/An-evolutionary-perspective-on-the-relationship
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectKinetochore
dc.subjectCENP-E
dc.subjectKinesin
dc.subjectChromosome
dc.subjectHolocentric
dc.subjectMitosis
dc.titleAn evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignmentpor
dc.titleAn evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignmentpor
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.issue24
oaire.citation.titleJournal of Cell Science
oaire.citation.volume137
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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