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An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment
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Orientador(es)
Resumo(s)
Chromosome alignment during mitosis can occur as a consequence of bi-orientation or is assisted by the CENP-E (kinesin-7) motor at kinetochores. We previously found that Indian muntjac chromosomes with larger kinetochores bi-orient more efficiently and are biased to align in a CENP-E-independent manner, suggesting that CENP-E dependence for chromosome alignment negatively correlates with kinetochore size. Here, we used targeted phylogenetic profiling of CENP-E in monocentric (localized centromeres) and holocentric (centromeres spanning the entire chromosome length) clades to test this hypothesis at an evolutionary scale. We found that, despite being present in common ancestors, CENP-E was lost more frequently in taxa with holocentric chromosomes, such as Hemiptera and Nematoda. Functional experiments in two nematodes with holocentric chromosomes in which a CENP-E ortholog is absent (Caenorhabditis elegans) or present (Pristionchus pacificus) revealed that targeted expression of human CENP-E to C. elegans kinetochores partially rescued chromosome alignment defects associated with attenuated polar-ejection forces, whereas CENP-E inactivation in P. pacificus had no detrimental effects on mitosis and viability. These data showcase the dispensability of CENP-E for mitotic chromosome alignment in species with larger kinetochores.
Descrição
Palavras-chave
Kinetochore CENP-E Kinesin Chromosome Holocentric Mitosis
Contexto Educativo
Citação
Almeida, A. C., Rocha, H., Raas, M. W. D., Witte, H., Sommer, R. J., Snel, B., Kops, G. J. P. L., Gassmann, R., & Maiato, H. (2024). An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment. Journal of Cell Science, 137(24), jcs263466. https://doi.org/10.1242/jcs.263466
Editora
The Company of Biologists