Publication
Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
| dc.contributor.author | Oliveira, Nélia | |
| dc.contributor.author | Costa-Rama, Estefanía | |
| dc.contributor.author | Viswanathan, Subramanian | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.contributor.author | Pereira, Lourdes | |
| dc.contributor.author | Morais, Simone | |
| dc.date.accessioned | 2019-09-13T09:10:09Z | |
| dc.date.available | 2019-09-13T09:10:09Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | This work describes a sensitive voltammetric immunosensor for label‐free electroanalysis of the prostate specific antigen (PSA), the main biomarker of prostate cancer. A gold electrode was firstly modified with the optimum self‐assembled monolayer (SAM), 1,6‐hexanedithiol, followed by the subsequent adsorption of gold nanoparticles (AuNPs) and, then, the monoclonal antibody to recognize PSA was immobilized. The influence of the most significant experimental variables (SAM type and incubation time, AuNPs deposition, antibody concentration and bovine serum albumin immobilization) on the biosensor response was studied by microscopy and voltammetry techniques. The electroanalytical detection was based on the interaction between PSA antibody and PSA via square‐wave voltammetry using ferrocyanide/ferricyanide as electrochemical redox indicator. Using the proposed immunosensor, PSA was specifically detected within the linear range between 0.2 and 200 ng mL−1 with 0.01 ng mL−1 as limit of detection. The immunosensor allows accurate, reproducible and sensitive (22.7 % reduction mL ng−1) detection in a concentration range useful for clinical purposes. | pt_PT |
| dc.description.sponsorship | The authors are grateful for financial support from theEuropean Union (FEDER funds through COMPETE)and National Funds (FCT) through projects UID/QUI/50006/2013 and UID/CTM/50011/2013. The authors alsothank financial support by Norte Portugal RegionalOperational Programme (NORTE 2020), under thePORTUGAL 2020 Partnership Agreement, through theEuropean Regional Development Fund (ERDF): projectsNorte-01-0145-FEDER-000011, Norte-01-0145-FEDER-000024 and POCI-01-0145-FEDER-007679. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1002/elan.201800417 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/14590 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Wiley-VCH Verlag | pt_PT |
| dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201800417 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Electrochemical immunosensor | pt_PT |
| dc.subject | Self-assembled monolayer | pt_PT |
| dc.subject | Gold nanoparticles | pt_PT |
| dc.subject | Cancer biomarker | pt_PT |
| dc.subject | Prostate specific antigen | pt_PT |
| dc.title | Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FCTM%2F50011%2F2013/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT | |
| oaire.citation.endPage | 2611 | pt_PT |
| oaire.citation.issue | 11 | pt_PT |
| oaire.citation.startPage | 2604 | pt_PT |
| oaire.citation.title | Electroanalysis | pt_PT |
| oaire.citation.volume | 30 | pt_PT |
| oaire.fundingStream | 5876 | |
| oaire.fundingStream | 5876 | |
| person.familyName | Delerue-Matos | |
| person.familyName | Morais | |
| person.givenName | Cristina | |
| person.givenName | Simone | |
| person.identifier | 1598822 | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.ciencia-id | B111-BBFB-F200 | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.orcid | 0000-0001-6433-5801 | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 6603741848 | |
| person.identifier.scopus-author-id | 7007053747 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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