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Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir

2011-09Journal article Open access
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dc.contributor.authorMartins, António
dc.contributor.authorMoreira, Cristiana
dc.contributor.authorVale, Micaela
dc.contributor.authorFreitas, Marisa
dc.contributor.authorRegueiras, Ana
dc.contributor.authorAntunes, Agostinho
dc.contributor.authorVasconcelos, Vítor
dc.date.accessioned2021-09-28T08:57:08Z
dc.date.available2021-09-28T08:57:08Z
dc.date.issued2011-09
dc.description.abstractBlooms of toxic cyanobacteria are becoming increasingly frequent, mainly due to water quality degradation. This work applied qPCR as a tool for early warning of microcystin(MC)-producer cyanobacteria and risk assessment of water supplies. Specific marker genes for cyanobacteria, Microcystis and MC-producing Microcystis, were quantified to determine the genotypic composition of the natural Microcystis population. Correlations between limnological parameters, pH, water temperature, dissolved oxygen and conductivity and MC concentrations as well as Microcystis abundance were assessed. A negative significant correlation was observed between toxic (with mcy genes) to non-toxic (without mcy genes) genotypes ratio and the overall Microcystis density. The highest proportions of toxic Microcystis genotypes were found 4-6 weeks before and 8-10 weeks after the peak of the bloom, with the lowest being observed at its peak. These results suggest positive selection of non-toxic genotypes under favorable environmental growth conditions. Significant positive correlations could be found between quantity of toxic genotypes and MC concentration, suggesting that the method applied can be useful to predict potential MC toxicity risk. No significant correlation was found between the limnological parameters measured and MC concentrations or toxic genotypes proportions indicating that other abiotic and biotic factors should be governing MC production and toxic genotypes dynamics. The qPCR method here applied is useful to rapidly estimate the potential toxicity of environmental samples and so, it may contribute to the more efficient management of water use in eutrophic systems.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMartins, A., Moreira, C., Vale, M., Freitas, M., Regueiras, A., Antunes, A., & Vasconcelos, V. (2011). Seasonal dynamics of Microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir. Marine Drugs, 9(10), 1715-1730. https://www.mdpi.com/1660-3397/9/10/1715pt_PT
dc.identifier.doi10.3390/md9101715pt_PT
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.22/18591
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1660-3397/9/10/1715pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicrocystinspt_PT
dc.subjectMicrocystispt_PT
dc.subjectqPCRpt_PT
dc.subjectWater quality monitoringpt_PT
dc.subjectWater managementpt_PT
dc.titleSeasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoirpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1730pt_PT
oaire.citation.startPage1715pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume9pt_PT
person.familyNameFreitas
person.givenNameMarisa
person.identifier.orcid0000-0002-8477-7241
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc51ee763-c229-4dba-a65e-4c4cd2f2d15f
relation.isAuthorOfPublication.latestForDiscoveryc51ee763-c229-4dba-a65e-4c4cd2f2d15f

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