Publication
Molecularly imprinted electrochemical sensor for the point-of-care detection of a breast cancer biomarker (CA 15-3)
| dc.contributor.author | Pacheco, João | |
| dc.contributor.author | Silva, Marta S.V. | |
| dc.contributor.author | Freitas, Maria | |
| dc.contributor.author | Nouws, Henri | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.date.accessioned | 2019-10-07T14:13:12Z | |
| dc.date.available | 2019-10-07T14:13:12Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | The incidence of breast cancer has been increasing over the years. To control and monitor this disease several tumor biomarkers have been proposed for early diagnosis, patient follow-up and/or treatment guidance. The only serum breast cancer biomarker in current use is the cancer antigen 15-3 (CA 15-3). In this work a molecularly imprinted polymer (MIP)-based electrochemical (voltammetric) sensor to monitor breast cancer was developed, based on direct surface imprinting of CA 15-3 on a screen-printed gold electrode (Au-SPE). The imprinting was performed in two steps: (1) adsorption of CA 15-3 on the surface of the Au-SPE and (2) electropolymerization of 2-aminophenol around the adsorbed protein. After extraction of the imprinted protein voltammetric analysis was conducted using hexacyanoferrate(II/III) as redox probe, measuring the signals before and after protein binding. The sensor was characterized by voltammetric techniques and electrochemical impedance spectroscopy, and the analytical responses of imprinted and non-imprinted polymer sensors were studied. A linear relationship between the peak current intensity of the redox probe and the logarithm of CA 15-3 concentration was established between 5 and 50 U mL−1, achieving a limit of detection of 1.5 U mL−1. The prepared MIP-sensor provides fast (15 min) analysis and is cheap, easy to prepare, disposable and could easily be integrated in small portable point-of care devices. | pt_PT |
| dc.description.sponsorship | João Paulo Grosso Pacheco is grateful to FCT-Fundação para a Ciência e a Tecnologia for a Pos-Doc. grant (SFRH/BPD/101419/2014), financed by POPH-QREN-Tipologia 4.1-Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. Maria Freitas is grateful to FCT (Fundação para a Ciência e a Tecnologia) for her PhD grant (SFRH/BD/111942/2015). This work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT) through project UID/QUI/50006/2013. The authors are greatly indebted to all financing sources. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1016/j.snb.2017.10.027 | |
| dc.identifier.issn | 0925-4005 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/14708 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation | Construction of point-of-care molecularly imprinted electrochemical devices for cancer biomarkers detection | |
| dc.relation | High-performance magnetic nanoparticle-based immunoassays for breast cancer biomarker analysis | |
| dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S092540051731907X?via%3Dihub | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Breast cancer | pt_PT |
| dc.subject | Molecularly imprinted polymer | pt_PT |
| dc.subject | CA 15-3 | pt_PT |
| dc.subject | Electrochemical sensor | pt_PT |
| dc.subject | Point-of-care analysis | pt_PT |
| dc.title | Molecularly imprinted electrochemical sensor for the point-of-care detection of a breast cancer biomarker (CA 15-3) | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Construction of point-of-care molecularly imprinted electrochemical devices for cancer biomarkers detection | |
| oaire.awardTitle | High-performance magnetic nanoparticle-based immunoassays for breast cancer biomarker analysis | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F101419%2F2014/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F111942%2F2015/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT | |
| oaire.citation.endPage | 912 | pt_PT |
| oaire.citation.startPage | 905 | pt_PT |
| oaire.citation.title | Sensors and Actuators B: Chemical | pt_PT |
| oaire.citation.volume | 256 | pt_PT |
| oaire.fundingStream | OE | |
| oaire.fundingStream | 5876 | |
| person.familyName | Pacheco | |
| person.familyName | Nouws | |
| person.familyName | Delerue-Matos | |
| person.givenName | João | |
| person.givenName | Hendrikus | |
| person.givenName | Cristina | |
| person.identifier | 1046746 | |
| person.identifier.ciencia-id | 8D13-F178-F44D | |
| person.identifier.ciencia-id | 9B1A-9CED-6EE3 | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.orcid | 0000-0001-8366-0490 | |
| person.identifier.orcid | 0000-0002-1472-8866 | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.rid | D-5249-2013 | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 35519962400 | |
| person.identifier.scopus-author-id | 7801450247 | |
| person.identifier.scopus-author-id | 6603741848 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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