A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection

Group B streptococcal (GBS) meningitis remains a devastating disease. The absence of an animal model reproducing the natural infectious process has limited our understanding of the disease and, consequently, delayed the development of effective treatments. We describe here a mouse model in which bacteria are transmitted to the offspring from vaginally colonised pregnant females, the natural route of infection. We show that GBS strain BM110, belonging to the CC17 clonal complex, is more virulent in this vertical transmission model than the isogenic mutant BM110∆cylE, which is deprived of hemolysin/cytolysin. Pups exposed to the more virulent strain exhibit higher mortality rates and lung inflammation than those exposed to the attenuated strain. Moreover, pups that survive to BM110 infection present neurological developmental disability, revealed by impaired learning performance and memory in adulthood. The use of this new mouse model, that reproduces key steps of GBS infection in newborns, will promote a better understanding of the physiopathology of GBS-induced meningitis.

Keywords

Animals Behavior, Animal Body Weight Hemolysin Proteins Inflammation Maze Learning Meningitis Meningitis, Bacterial Mice Mice, Inbred BALB C Perforin Pregnancy Pregnancy, Animal Streptococcal Infections Streptococcus agalactiae Disease Models, Animal Infectious Disease Transmission, Vertical

URI

http://hdl.handle.net/10400.22/13734

Citation

Andrade, E.B., Magalhães, A., Puga, A. et al. A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection (2018). Nat Commun 9, 3138. https://doi.org/10.1038/s41467-018-05492-y