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Abnormal immunoreactivity to serotonin in cerebellar purkinje cells after neonatal cocaine exposure

2004Journal article Open access
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dc.contributor.authorSummavielle, Teresa
dc.contributor.authorAlves, Cecília J.
dc.contributor.authorMonteiro, Pedro
dc.contributor.authorTavares, Maria Amélia
dc.date.accessioned2024-05-09T08:36:29Z
dc.date.available2024-05-09T08:36:29Z
dc.date.issued2004
dc.description.abstractNeonatal cocaine is known to affect the developing serotonergic system in many brain structures, including the cerebellum. Changes in the cerebellar Purkinje cells after drug exposure are well documented and result in impairment of movement and other cerebellar disorders such as ataxia. These cells have a major postnatal developmental pattern; therefore, neonatal exposure to cocaine is likely to affect them. In this work, male and female Wistar rats were injected with 15 mg of cocaine hydrochloride/kg body weight/day, subcutaneously, in two daily doses, from postnatal day 1 (PND1) to PND29. Controls were given 0.9% of saline. On PND14, PND21, and PND30, rats were transcardially perfused, and brains removed and cryoprotected. Coronal sections from the cerebellum were processed for immunocytochemistry of cells containing serotonin (5-hydroxytryptamine, or 5-HT). At the same postnatal age, rats from at least three different litters were sacrificed by decapitation, and brains were dissected for determination of 5-HT in the cerebellum by high-performance liquid chromatography with electrochemical detection. Upon the expected distribution of immunoreactivity to 5-HT, an abnormal immunoreactivity to 5-HT was observed in the Purkinje cells of six cocaineexposed animals, but not in control animals. Also, levels of cerebellar 5-HT in cocaine-exposed rats were significantly increased on PND21. These results, together with previously reported observations of altered patterns of motor behavior, indicate that neonatal cocaine exposure affects the serotonergic cerebellar system, altering the standard development of Purkinje cells and possibly compromising the motor function.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSummavielle, T., Alves, C. J., Monteiro, P. R. r., & Tavares, M. A. (2004). Abnormal immunoreactivity to serotonin in cerebellar purkinje cells after neonatal cocaine exposure. Annals of the New York Academy of Sciences, 1025(1), 630–637. https://doi.org/10.1196/annals.1316.078pt_PT
dc.identifier.doi10.1196/annals.1316.078pt_PT
dc.identifier.eissn1749-6632
dc.identifier.issn0077-8923
dc.identifier.urihttp://hdl.handle.net/10400.22/25487
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationThis work was supported by project PRAXIS/PSAU/P/ SAU/8/96, by project POCTI/ESP/43630/1999, and by Programa de Financiamento Plurianual do IBMC. Teresa Summavielle received a Ph.D. grant from Fundação para a Ciência e Tecnologia (PRAXIS/BD/14742/97), and Cecília J. Alves a research grant from Fundação para a Ciência e Tecnologia (POCTI/43630-pr 381101).pt_PT
dc.relation.publisherversionhttps://nyaspubs.onlinelibrary.wiley.com/doi/10.1196/annals.1316.078pt_PT
dc.subjectCocaine Serotonin (5-hydroxytryptamine, or 5-HT)pt_PT
dc.subjectNeonatalpt_PT
dc.subjectCerebellumcpt_PT
dc.subjectPurkinje cellspt_PT
dc.subjectRatpt_PT
dc.titleAbnormal immunoreactivity to serotonin in cerebellar purkinje cells after neonatal cocaine exposurept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage637pt_PT
oaire.citation.startPage630pt_PT
oaire.citation.titleAnnals of the New York Academy of Sciencespt_PT
oaire.citation.volume1025 (1)pt_PT
person.familyNameSummavielle
person.familyNameMonteiro
person.givenNameTeresa
person.givenNamePedro
person.identifier677706
person.identifier.ciencia-idC41E-0816-5C85
person.identifier.ciencia-id2414-4468-928B
person.identifier.orcid0000-0003-2548-6281
person.identifier.orcid0000-0002-3991-9191
person.identifier.ridC-9776-2012
person.identifier.scopus-author-id6603092949
person.identifier.scopus-author-id6504494827
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication207ee2de-85a0-4144-9e7e-b376c600e065
relation.isAuthorOfPublicationfae8b5b3-db39-4d56-a2b1-2db0388279b9
relation.isAuthorOfPublication.latestForDiscovery207ee2de-85a0-4144-9e7e-b376c600e065

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