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Effect of Adipocyte Secretome in Melanoma Progression and Vasculogenic Mimicry

2016Journal article Open access
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dc.contributor.authorCoelho, Pedro
dc.contributor.authorAlmeida, Joana
dc.contributor.authorPrudêncio, Cristina
dc.contributor.authorFernandes, Rúben
dc.contributor.authorSoares, Raquel
dc.date.accessioned2019-07-01T17:04:20Z
dc.date.available2019-07-01T17:04:20Z
dc.date.issued2016
dc.description.abstractObesity, favored by the modern lifestyle, acquired epidemic proportions nowadays. Obesity has been associated with various major causes of death and morbidity including malignant neoplasms. This increased prevalence has been accompanied by a worldwide increase in cutaneous melanoma incidence rates during the last decades. Obesity involvement in melanoma aetiology has been recognized, but the implicated mechanisms remain unclear. In the present study, we address this relationship and investigate the influence of adipocytes secretome on B16-F10 and MeWo melanoma cell lines. Using the 3T3-L1 adipocyte cell line, as well as ex vivo subcutaneous (SAT) and visceral (VAT) adipose tissue conditioned medium, we were able to show that adipocyte-released factors play a dual role in increasing melanoma cell overall survival, both by enhancing proliferation and decreasing apoptosis. B16-F10 cell migration and cell-cell and cell-matrix adhesion capacity were predominantly enhanced in the presence of SAT and VAT released factors. Melanocytes morphology and melanin content were also altered by exposure to adipocyte conditioned medium disclosing a more dedifferentiated phenotype of melanocytes. In addition, exposure to adipocyte-secreted molecules induced melanocytes to rearrange, on 3D cultures, into vessel-like structures, and generate characteristic vasculogenic mimicry patterns. These findings are corroborated by the released factors profile of 3T3-L1, SAT, and VAT assessed by microarrays, and led us to highlight the mechanisms by which adipose secretome from sub-cutaneous or visceral depots promote melanoma progression.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCoelho, P., Almeida, J., Prudêncio, C., Fernandes, R., & Soares, R. (2016). Effect of Adipocyte Secretome in Melanoma Progression and Vasculogenic Mimicry. Journal of Cellular Biochemistry, 117(7), 1697–1706. https://doi.org/10.1002/jcb.25463
dc.identifier.doi10.1002/jcb.25463pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14212
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationPEst‐OE/SAU/UI0038/2011pt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/jcb.25463pt_PT
dc.subject3T3-L1 Cellspt_PT
dc.subjectAdipocytespt_PT
dc.subjectCell Dedifferentiationpt_PT
dc.subjectCell Line, Tumorpt_PT
dc.subjectIntra-Abdominal Fatpt_PT
dc.subjectMelanocytespt_PT
dc.subjectMelanomapt_PT
dc.subjectNeovascularization, Pathologicpt_PT
dc.subjectObesitypt_PT
dc.subjectSubcutaneous Fatpt_PT
dc.titleEffect of Adipocyte Secretome in Melanoma Progression and Vasculogenic Mimicrypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04293%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F80434%2F2011/PT
oaire.citation.endPage1706pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1697pt_PT
oaire.citation.titleJournal of Cellular Biochemistrypt_PT
oaire.citation.volume117pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameCoelho
person.familyNamePrudêncio
person.familyNameFernandes
person.givenNamePedro
person.givenNameCristina
person.givenNameRúben
person.identifier1200571
person.identifier635792
person.identifier.ciencia-idBE18-3DB2-8D79
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.orcid0000-0002-2343-1108
person.identifier.orcid0000-0002-9920-936X
person.identifier.orcid0000-0001-8933-3984
person.identifier.ridAAZ-9239-2020
person.identifier.scopus-author-id57214054449
person.identifier.scopus-author-id6508057930
person.identifier.scopus-author-id57640135700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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