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Microwave- and Ultrasound-Assisted Extraction of Cucurbita pepo Seeds: A Comparison Study of Antioxidant Activity, Phenolic Profile, and In-Vitro Cells Effects

2022Journal article Open access
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dc.contributor.authorMacedo, Catarina
dc.contributor.authorSilva, Ana Margarida
dc.contributor.authorFerreira, Ana Sofia
dc.contributor.authorMoreira, Manuela M.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorRodrigues, Francisca
dc.date.accessioned2023-01-24T11:50:37Z
dc.date.available2023-01-24T11:50:37Z
dc.date.issued2022
dc.description.abstractNowadays there is a growing demand for nutraceuticals to prevent diseases related to redox imbalances, such as atherosclerosis and diabetes, being crucial to search for new matrixes rich in bioactive compounds. This work aims to characterize the value-added compounds extracted from Curcubita pepo seeds using green methodologies, namely microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE), employing water as an extracting solvent for two ratios (condition 1: 1 mg/20 mL; condition 2: 2.5 mg/20 mL). The extract with the best antioxidant/antiradical activity in FRAP (71.09 μmol FSE/g DW) and DPPH (5.08 mg TE/g DW) assays was MAE condition 1, while MAE condition 2 exhibited the highest activity in the ABTS assay (13.29 mg AAE/g DW) and TPC (16.89 mg GAE/g DW). A remarkable scavenging capacity was observed, particularly for HOCl, with IC50 values ranging from 1.88–13.50 μg/mL. A total of 21 phenolic compounds were identified, being catechin (4.567–7.354 mg/g DW), caffeine (1.147–2.401 mg/g DW) and gallic acid (0.945–1.337 mg/g DW) predominant. No adverse effects were observed on Caco-2 viability after exposure to MAE extracts, while the other conditions led to a slight viability decrease in NSC-34. These results highlighted that the extract from MAE condition 2 is the most promising as a potential nutraceutical ingredient.pt_PT
dc.description.sponsorshipThis research was funded by UIDB/50006/2020 and UIDP/50006/2020 by the Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through national funds. Ana Margarida Silva (SFRH/BD/144994/2019) and Ana Sofia Ferreira (2020.07519.BD) are thankful for the Ph.D. grants financed by POPH‐QREN and subsidized by the European Science Foundation and Ministério da Ciência, Tecnologia e Ensino Superior. Manuela M. Moreira (CEECIND/02702/2017) and Francisca Rodrigues (CEECIND/01886/2020) are thankful for their contracts financed by FCT/MCTES—CEEC Individual Program Contract.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/app12031763pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21818
dc.language.isoengpt_PT
dc.publisherMDPIpt_PT
dc.relationCEECIND/01886/2020pt_PT
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationFrom soil to skin: Eco-friendly extract from kiwiberry leaves for prevention of skin aging process
dc.relationEco-innovative strategy to valorize Chestnut Castanea sativa Shells as Active Ingredient for Oral Films: A New Approach to Prevent/Treat Oral Mucositis
dc.relationNot Available
dc.relation.publisherversionhttps://www.mdpi.com/2076-3417/12/3/1763pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCurcubita pepo seedspt_PT
dc.subjectGreen extraction techniquespt_PT
dc.subjectNutraceutical industrypt_PT
dc.subjectValorizationpt_PT
dc.subjectSustainabilitypt_PT
dc.titleMicrowave- and Ultrasound-Assisted Extraction of Cucurbita pepo Seeds: A Comparison Study of Antioxidant Activity, Phenolic Profile, and In-Vitro Cells Effectspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleFrom soil to skin: Eco-friendly extract from kiwiberry leaves for prevention of skin aging process
oaire.awardTitleEco-innovative strategy to valorize Chestnut Castanea sativa Shells as Active Ingredient for Oral Films: A New Approach to Prevent/Treat Oral Mucositis
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F144994%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07519.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F02702%2F2017%2FCP1427%2FCT0004/PT
oaire.citation.issue3pt_PT
oaire.citation.startPage1763pt_PT
oaire.citation.titleApplied Sciencespt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
oaire.fundingStreamCEEC IND 2017
person.familyNamePinheiro Macedo
person.familyNameSilva
person.familyNameFerreira
person.familyNameMoreira
person.familyNameDelerue-Matos
person.familyNamePinto Lisboa Martins Rodrigues Sarmento
person.givenNameCatarina
person.givenNameAna Margarida
person.givenNameAna Sofia
person.givenNameManuela M.
person.givenNameCristina
person.givenNameFrancisca
person.identifier1552042
person.identifier2496526
person.identifier.ciencia-id6F19-BED8-EEC7
person.identifier.ciencia-id5C16-8C22-EF1E
person.identifier.ciencia-idA414-640D-9066
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.ciencia-id4F19-3D98-2CBA
person.identifier.orcid0000-0002-6108-8582
person.identifier.orcid0000-0002-1823-9816
person.identifier.orcid0000-0001-5268-4932
person.identifier.orcid0000-0003-1500-9211
person.identifier.orcid0000-0002-3924-776X
person.identifier.orcid0000-0001-8803-0041
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id57203495060
person.identifier.scopus-author-id7103289231
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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