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Identification of fecal biomarkers for early detection of pre- and cancerous colorectal lesions

2023-11-23Master thesis Embargoed access
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dc.contributor.advisorLima, Luís
dc.contributor.advisorSantos, Marlene
dc.contributor.authorSantos, Daniela Alexandra Rocha
dc.date.accessioned2024-01-29T14:59:49Z
dc.date.embargo2026-11-23
dc.date.issued2023-11-23
dc.description.abstractColorectal cancer (CRC) is highly prevalent in Portugal, and early detection significantly improves survival rates. However, current CRC screening methods have limitations, making necessary to develop new tools. MicroRNAs (miRs) exhibit altered expression in tumors and can be detected in biological samples, such as blood and stool, posing as a promising CRC screening tool. Identify stool-based miR biomarkers to enhance CRC screening. After a literature review, eight miRs were selected miRs and evaluated in stool samples on a dataset of 96 individuals who underwent a colonoscopy, using quantitative real-time PCR. Seven miRs were detected in stool samples, miR-451 demonstrated a 63.16% sensitivity (AUC: 0.707) for high-grade dysplasia (HGD), while miR-199a-5p exhibited 70.83% sensitivity (AUC: 0.648) for advanced lesions detection. Our predictive model combining miR-199a-5p, miR-21-5p and miR-451 achieved 83.33% sensitivity and 69.57% specificity for HGD discrimination. Moreover, a combination of miR-21-5p and miR-199a-5p demonstrate a good performance for CRC (AUC: 0.724) and Advanced Lesions identification (AUC: 0.724), with high sensitivity (87.50% for CRC and 77.05% for Advanced lesions identification). Combinations of miR-21-5p, miR-199a-5p and miR-451 show promise for improving CRC screening. However, further studies are needed to validate these findings.pt_PT
dc.identifier.tid203473892pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/24800
dc.language.isoengpt_PT
dc.subjectMicroRNAspt_PT
dc.subjectColorectal cancerpt_PT
dc.subjectBiomarkerpt_PT
dc.subjectScreeningpt_PT
dc.subjectEarly detectionpt_PT
dc.titleIdentification of fecal biomarkers for early detection of pre- and cancerous colorectal lesionspt_PT
dc.typemaster thesis
dspace.entity.typePublication
rcaap.rightsembargoedAccesspt_PT
rcaap.typemasterThesispt_PT
thesis.degree.nameFarmáciapt_PT

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