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Protein imprinted materials designed with charged binding sites on screen-printed electrode for microseminoprotein-beta determination in biological samples

2016Journal article Restricted access
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dc.contributor.authorRebelo, Tânia S.C.R.
dc.contributor.authorPereira, Carlos M.
dc.contributor.authorSales, Goreti
dc.contributor.authorNoronha, J.P.
dc.contributor.authorSilva, Fernando
dc.date.accessioned2017-07-13T10:56:43Z
dc.date.embargo2117
dc.date.issued2016
dc.description.abstractIn the past few years a large effort is being made aiming at the development of fast and reliable tests for cancer biomarkers. Protein imprinted sensors can be a fast and reliable strategy to develop tailor made sensors for a large number of relevant molecules. This work aims to produce, optimize and use in biological samples a biosensor for microseminoprotein-beta (MSMB). Caffeic acid (CAF) electropolimerization was performed in the presence of microseminoprotein-beta (MSMB) creating target protein specific cavities on the surface of a screen-printed carbon. Dopamine was introduced as charged monomer labelling the binding site and was allowed to self-organize around the protein. The subsequent electropolimerization was made by applying a constant potential of +2.0 V, for 30 s, on a carbon screen-printed electrode, immersed in a solution of protein and CAF prepared in phosphate buffer. The sensor with charged monomers showed a more sensitive response, with an average slope of−7.59 A/decade, linear concentration range of 0.5–100 ng/mL and a detection limit of 0.12 ng/mL. The corresponding non-imprinted sensor displayed an inconsistent response over the range of the calibration curve. The biosensor was successfully applied to the analysis of MSMB in serum and urine samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2015.09.133pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/10036
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesSensors and Actuators B: Chemical;Vol. 223
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S092540051530441Xpt_PT
dc.subjectMicroseminoprotein-betapt_PT
dc.subjectCaffeic acidpt_PT
dc.subjectElectrochemical biosensorpt_PT
dc.subjectProtein imprinted materialspt_PT
dc.subjectScreen-printed electrodespt_PT
dc.subjectUrinept_PT
dc.subjectSerumpt_PT
dc.titleProtein imprinted materials designed with charged binding sites on screen-printed electrode for microseminoprotein-beta determination in biological samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
person.familyNameFerreira Sales
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id7005174717
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isAuthorOfPublication.latestForDiscoveryb6d3a2d0-608a-4a92-834b-8d7b29069fef

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