| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 988.56 KB | Adobe PDF |
Orientador(es)
Resumo(s)
In the past few years a large effort is being made aiming at the development of fast and reliable tests for cancer biomarkers. Protein imprinted sensors can be a fast and reliable strategy to develop tailor made sensors for a large number of relevant molecules.
This work aims to produce, optimize and use in biological samples a biosensor for microseminoprotein-beta (MSMB).
Caffeic acid (CAF) electropolimerization was performed in the presence of microseminoprotein-beta (MSMB) creating target protein specific cavities on the surface of a screen-printed carbon. Dopamine was introduced as charged monomer labelling the binding site and was allowed to self-organize around the protein. The subsequent electropolimerization was made by applying a constant potential of +2.0 V, for 30 s, on a carbon screen-printed electrode, immersed in a solution of protein and CAF prepared in phosphate buffer.
The sensor with charged monomers showed a more sensitive response, with an average slope of−7.59 A/decade, linear concentration range of 0.5–100 ng/mL and a detection limit of 0.12 ng/mL. The corresponding non-imprinted sensor displayed an inconsistent response over the range of the calibration curve. The biosensor was successfully applied to the analysis of MSMB in serum and urine samples.
Descrição
Palavras-chave
Microseminoprotein-beta Caffeic acid Electrochemical biosensor Protein imprinted materials Screen-printed electrodes Urine Serum
Contexto Educativo
Citação
Editora
Elsevier