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Electrochemical sensing of the thyroid hormone thyronamine (T0AM) via molecular imprinted polymers (MIPs)

2019-03Journal article Open access
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dc.contributor.authorPacheco, João
dc.contributor.authorRebelo, Patrícia
dc.contributor.authorCagide, Fernando
dc.contributor.authorGonçalves, Luís Moreira
dc.contributor.authorBorges, Fernanda
dc.contributor.authorRodrigues, José António
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2020-07-10T14:47:17Z
dc.date.available2020-07-10T14:47:17Z
dc.date.issued2019-03
dc.description.abstractRecent studies have shown that besides the well-known T3 (triiodothyronine) and T4 (thyroxine) there might be other important thyroid hormones, in particular T0AM (thyronamine) and T1AM (3-iodothyronamine). The absence of a large number of studies showing their precise importance might be explained by the limited number of analytical methodologies available. This work aims to show an electroanalytical alternative making use of electropolymerized molecularly imprinted polymer (MIPs). The MIPs' polymerization is performed on the surface of screen-printed carbon electrodes (SPCEs), using 4-aminobenzoic acid (4-ABA) as the building and functional monomer and the analyte T0AM as the template. The step-by-step construction of the SPCE-MIP sensor was studied by cyclic voltammetry (CV) and by electrochemical impedance spectroscopy (EIS). After optimization, by means of square-wave voltammetry, the SPCE-MIP showed suitable selectivity (in comparison with other thyroid hormones and catechol amines), repeatability (intra-day of 3.9%), a linear range up to 10 μmol L-1 (0.23 × 103 μg dL-1) with an r2 of 0.998 and a limit of detection (LOD) and quantification (LOQ) of 0.081 and 0.27 μmol L-1 (1.9 and 6.2 μg dL-1), respectively.pt_PT
dc.description.sponsorshipThis project was supported by Fundação para a Ciência e a Tecnologia (FCT), and FEDER/COMPETE (UID/QUI/00081/2013, UID/QUI/50006/2013, POCI-01-0145-FEDER-006980 and NORTE-01-0145-FEDER-000028). JGP (SFRH/BPD/101419/2014), PR (SFRH/BD/132384/2017) and LMG (SFRH/BPD/76544/2011) wish to acknowledge FCT for their research grants. FC thanks NORTE-01-0145-FEDER-000028 grant.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.talanta.2018.10.090pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16108
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0039914018311366?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCarbonpt_PT
dc.subjectElectrochemistrypt_PT
dc.subjectElectrodespt_PT
dc.subjectPolymerizationpt_PT
dc.subjectPolymerspt_PT
dc.subjectPrintingpt_PT
dc.subjectSurface Propertiespt_PT
dc.subjectThyroninespt_PT
dc.subjectMolecular Imprintingpt_PT
dc.titleElectrochemical sensing of the thyroid hormone thyronamine (T0AM) via molecular imprinted polymers (MIPs)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00081%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.endPage696pt_PT
oaire.citation.startPage689pt_PT
oaire.citation.titleTalantapt_PT
oaire.citation.volume194pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNamePacheco
person.familyNameRebelo
person.familyNameDelerue-Matos
person.givenNameJoão
person.givenNamePatrícia
person.givenNameCristina
person.identifier.ciencia-id8D13-F178-F44D
person.identifier.ciencia-id6112-1293-B25E
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-8366-0490
person.identifier.orcid0000-0001-5209-668X
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id35519962400
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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