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Prevalence of antibiotic resistance genes in multidrug-resistant enterobacteriaceae on Portuguese Livestock Manure

2019-03Journal article Open access
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dc.contributor.authorAmador, Paula
dc.contributor.authorFernandes, Rúben
dc.contributor.authorPrudêncio, Cristina
dc.contributor.authorDuarte, Isabel
dc.date.accessioned2021-10-19T13:53:25Z
dc.date.available2021-10-19T13:53:25Z
dc.date.issued2019-03
dc.description.abstractThe exposure of both crop fields and humans to antibiotic-resistant bacteria in animal excreta is an emergent concern of the One Health initiative. This study assessed the contamination of livestock manure from poultry, pig, dairy farms and slaughterhouses in Portugal with resistance determinants. The resistance profiles of 331 Enterobacteriaceae isolates to eight β-lactam (amoxicillin, cefoxitin, cefotaxime, cefpirome, aztreonam, ceftazidime, imipenem and meropenem) and to five non-β-lactam antibiotics (tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ciprofloxacin (CIP), chloramphenicol (CHL) and gentamicin) was investigated. Forty-nine integron and non-β-lactam resistance genes were also screened for. Rates of resistance to the 13 antibiotics ranged from 80.8% to 0.6%. Multidrug resistance (MDR) rates were highest in pig farm samples (79%). Thirty different integron and resistance genes were identified. These were mainly associated with resistance to CHL (catI and catII), CIP (mainly, qnrS, qnrB and oqx), TET (mainly tet(A) and tet(M)) and SXT (mostly dfrIa group and sul3). In MDR isolates, integron presence and non-β-lactam resistance to TET, SXT and CHL were positively correlated. Overall, a high prevalence of MDR Enterobacteriaceae was found in livestock manure. The high gene diversity for antibiotic resistance identified in this study highlights the risk of MDR spread within the environment through manure use.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmador, P., Fernandes, R., Prudêncio, C., & Duarte, I. (2019). Prevalence of Antibiotic Resistance Genes in Multidrug-Resistant Enterobacteriaceae on Portuguese Livestock Manure. Antibiotics, 8(1), 23. https://www.mdpi.com/2079-6382/8/1/23pt_PT
dc.identifier.doi10.3390/antibiotics8010023pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.22/18738
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/8/1/23pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntibiotic-resistance genespt_PT
dc.subjectMultidrug resistancept_PT
dc.subjectLivestockpt_PT
dc.subjectAnimal excretapt_PT
dc.subjectEnvironmental contaminantspt_PT
dc.titlePrevalence of antibiotic resistance genes in multidrug-resistant enterobacteriaceae on Portuguese Livestock Manurept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage18pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume8pt_PT
person.familyNameFernandes
person.familyNamePrudêncio
person.givenNameRúben
person.givenNameCristina
person.identifier635792
person.identifier1200571
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.orcid0000-0001-8933-3984
person.identifier.orcid0000-0002-9920-936X
person.identifier.scopus-author-id57640135700
person.identifier.scopus-author-id6508057930
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication917f7713-0a35-4ef0-ae56-0784f7fc2187
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication.latestForDiscovery881a8ad5-ab13-4e49-89f4-08ca61cc81e3

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