Influence of puncture devices on the accuracy of cyclophosphamide dosing for chemotherapy administration

Carvalho, Susana; Cardoso, Andreia; Ferreira, Débora; Silva, Diana Dias da; Moreira, Fernando; Dias da Silva, Diana Cristina

Publication

Influence of puncture devices on the accuracy of cyclophosphamide dosing for chemotherapy administration

2025-06-12Research article

dc.contributor.author	Carvalho, Susana
dc.contributor.author	Cardoso, Andreia
dc.contributor.author	Ferreira, Débora
dc.contributor.author	Silva, Diana Dias da
dc.contributor.author	Moreira, Fernando
dc.contributor.author	Moreira, Fernando
dc.contributor.author	Dias da Silva, Diana Cristina
dc.date.accessioned	2025-10-02T08:56:46Z
dc.date.available	2025-10-02T08:56:46Z
dc.date.issued	2025-06-12
dc.description.abstract	Cyclophosphamide is one of the most commonly used cytotoxic drugs in chemotherapy protocols. Its preparation in the hospital setting involves handling concentrated solutions, which pose occupational exposure risks and potential variations in the final dose administered. The aim of this study was to evaluate the effect of aspiration devices on the concentration of cyclophosphamide in reconstituted solutions. An analytical method was validated using high-performance liquid chromatography coupled to a diode-array detector (HPLC-DAD) for quality control. Cyclophosphamide solutions were prepared and aspirated using either a conventional needle or spike device with or without a filtration system. The validated method demonstrated linearity (R2 = 0.9999), high precision (0.22–4.59%) and accuracy (88.9–99.4%), with a limit of quantification of 4.03 µg/mL. Significant differences (p < 0.001) were observed between samples aspirated with a needle and those aspirated with a spike fitted with a 5 µm filter, with the latter showing lower cyclophosphamide concentrations, suggesting partial retention of the drug. No significant differences were found between the needle and filterless spike preparations. These results suggest that the choice of aspiration device influences the final drug concentration, potentially affecting therapeutic efficacy. Standardisation of preparation techniques and an awareness of device limitations are essential to ensure accurate chemotherapy dosing and patient safety.	por
dc.identifier.citation	Carvalho, S., Cardoso, A., Ferreira, D., Dias da Silva, D., & Moreira, F. (2025). Influence of puncture devices on the accuracy of cyclophosphamide dosing for chemotherapy administration. Pharmaceuticals, 18(6), 879. https://doi.org/10.3390/ph18060879
dc.identifier.doi	https://www.mdpi.com/1424-8247/18/6/879
dc.identifier.eissn	1424-8247
dc.identifier.uri	http://hdl.handle.net/10400.22/30533
dc.language.iso	eng
dc.peerreviewed	yes
dc.publisher	MDPI
dc.relation	UID/50006
dc.relation.hasversion	https://www.mdpi.com/1424-8247/18/6/879
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	High-performance liquid chromatography with diode-array detection (HPLC-DAD)
dc.subject	Cyclophosphamide
dc.subject	Anticancer drugs
dc.subject	Chemotherapy preparation
dc.subject	Aspiration devices
dc.subject	Quality control
dc.subject	Dose accuracy
dc.title	Influence of puncture devices on the accuracy of cyclophosphamide dosing for chemotherapy administration	por
dc.type	research article
dspace.entity.type	Publication
oaire.citation.title	Pharmaceuticals
oaire.citation.volume	18
oaire.version	http://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyName	Moreira
person.familyName	Dias da Silva
person.givenName	Fernando
person.givenName	Diana Cristina
person.identifier	2947760
person.identifier.ciencia-id	5F16-AB92-94D0
person.identifier.ciencia-id	7715-CF06-F0B5
person.identifier.orcid	0000-0002-3452-1459
person.identifier.orcid	0000-0002-7331-9157
relation.isAuthorOfPublication	81213e80-09a5-4655-93dc-e54199edb2fe
relation.isAuthorOfPublication	1f32faf1-efa5-4ea0-982d-a755e1940abf
relation.isAuthorOfPublication.latestForDiscovery	81213e80-09a5-4655-93dc-e54199edb2fe