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Fluorometric method based on molecular recognition solid-phase extraction for determination of riboflavin in milk and infant formula

2016Journal article Open access
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dc.contributor.authorOsório, Marcelo V.
dc.contributor.authorMarques, Sara S.
dc.contributor.authorOliveira, Hugo M.
dc.contributor.authorBarreiros, Luisa
dc.contributor.authorSegundo, Marcela A.
dc.date.accessioned2019-07-03T16:41:45Z
dc.date.available2019-07-03T16:41:45Z
dc.date.issued2016
dc.description.abstractRiboflavin (vitamin B2) is involved in several biological processes, particularly in energy production, and it is acquired from food ingestion, principally from supplemented food during the first years of life. Therefore, a simple, fast and cost-effective high-throughput method for determination of riboflavin in milk and infant formula is proposed, based on selective extraction using commercially available molecularly imprinted polymers targeted to riboflavin, followed by direct fluorometric determination. Several aspects were studied, namely microplate assay conditions, the composition of eluting solution and the stability of riboflavin in the eluate. Hence, elution using 1% (v/v) acetic acid in methanol or in acetonitrile is recommended, followed by immediate analysis or solvent evaporation, with reconstitution and analysis within 24 h. The proposed method provided a LOD of 0.03 mg L−1, with working range for undiluted samples between 0.125 and 2 mg L−1, and sample throughput of 24 h−1. It was successfully applied to certified reference material NIST-1846 and also to commercial milk and infant formula samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jfca.2015.10.007pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14243
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAUTOMATION OF BIOCHEMICAL ASSAYS USING LAB-ON-VALVE AND BEAD INJECTION TECHNIQUES
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0889157515002306pt_PT
dc.subjectSolid-phase extractionpt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectRiboflavinpt_PT
dc.subjectHigh-throughput analysispt_PT
dc.subjectMicroplatept_PT
dc.subjectFluorimetrypt_PT
dc.subjectFood compositionpt_PT
dc.titleFluorometric method based on molecular recognition solid-phase extraction for determination of riboflavin in milk and infant formulapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAUTOMATION OF BIOCHEMICAL ASSAYS USING LAB-ON-VALVE AND BEAD INJECTION TECHNIQUES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F75065%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F89668%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.endPage146pt_PT
oaire.citation.startPage141pt_PT
oaire.citation.titleJournal of Food Composition and Analysispt_PT
oaire.citation.volume45pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameBarreiros
person.givenNameLuisa
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id6508205485
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57
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