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Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry

2020-09Journal article Restricted access
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dc.contributor.authorBarreiros, Luisa
dc.contributor.authorSilva, Eduarda M P
dc.contributor.authorAlencastre, Inês S
dc.contributor.authorLamghari, Meriem
dc.contributor.authorSegundo, Marcela A
dc.date.accessioned2020-09-14T10:02:41Z
dc.date.available2020-09-14T10:02:41Z
dc.date.issued2020-09
dc.description.abstractNeuropeptide Y (NPY) is a peptide widely distributed throughout the body that is involved in various physiological processes, including the regulation of feeding behavior and energy homeostasis. 5-Carbamimidamido-2-(2,2-diphenylacetamido)-N-[(4-hydroxyphenyl)methyl]pentanamide (BIBP 3226) is a selective NPY Y1 receptor antagonist with recognized application in bone regeneration studies, requiring quantification at picogram levels. Hence, BIBP 3226 determination is proposed here by a validated HPLC-MS/MS method, based on a reversed-phase Kinetex® core-shell C8 column (2.6 μm, 150 × 2.1 mm) at 30 °C, elution in isocratic mode using a mixture of acetonitrile and water (30:70, v/v), containing 0.1% (v/v) formic acid, at 0.25 mL min-1, detection in positive ionization mode, and data acquisition in selected reaction monitoring mode. Calibration curves were linear for concentrations ranging from 0.25 to 30 ng mL-1 with LOD and LOQ values as low as 0.1 and 0.3 pg in cell extracts and 16 and 48 pg in supernatant culture media, respectively. BIBP 3226 was successfully determined in cell extracts and supernatants obtained from internalization assays. Using similar exposure conditions, the amount of BIBP 3226 found in breast cancer cells (MCF7) was 72 to 657 times higher than that found in bone marrow cells (Wt C57BL/6 mice), providing an indirect indicator of NPY Y1 receptor expression.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00216-020-02825-zpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16247
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationPOCI-01-0145-FEDER-007274pt_PT
dc.relationPTDC/BIMMED/ 4041/2014pt_PT
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs00216-020-02825-zpt_PT
dc.subjectNeuropeptide Ypt_PT
dc.subjectY1 receptor antagonistpt_PT
dc.subjectBIBP 3226pt_PT
dc.subjectMass spectrometrypt_PT
dc.subjectReceptor expressionpt_PT
dc.titleDetermination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometrypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.citation.issue24pt_PT
oaire.citation.startPage6625-6632pt_PT
oaire.citation.titleAnalytical and Bioanalytical Chemistrypt_PT
oaire.citation.volume412pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBarreiros
person.givenNameLuisa
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id6508205485
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isProjectOfPublicationfc4a587f-c16e-4777-9549-2da7a1cf7d0e
relation.isProjectOfPublication.latestForDiscoveryfc4a587f-c16e-4777-9549-2da7a1cf7d0e

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