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ß-lactamases in the biochemistry and molecular biology laboratory

2009Journal article Open access
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dc.contributor.authorAmador, Paula
dc.contributor.authorPrudêncio, Cristina
dc.contributor.authorVieira, Mónica
dc.contributor.authorFerraz, Ricardo
dc.contributor.authorFonte, Rosália
dc.contributor.authorSilva, Nuno
dc.contributor.authorCoelho, Pedro
dc.contributor.authorFernandes, Rúben
dc.date.accessioned2014-02-03T12:05:13Z
dc.date.available2014-02-03T12:05:13Z
dc.date.issued2009
dc.date.updated2013-07-25T21:06:18Z
dc.description.abstractβ-lactamases are hydrolytic enzymes that inactivate the β-lactam ring of antibiotics such as penicillins and cephalosporins. The major diversity of studies carried out until now have mainly focused on the characterization of β-lactamases recovered among clinical isolates of Gram-positive staphylococci and Gram-negative enterobacteria, amongst others. However, only some studies refer to the detection and development of β-lactamases carriers in healthy humans, sick animals, or even in strains isolated from environmental stocks such as food, water, or soils. Considering this, we proposed a 10-week laboratory programme for the Biochemistry and Molecular Biology laboratory for majors in the health, environmental, and agronomical sciences. During those weeks, students would be dealing with some basic techniques such as DNA extraction, bacterial transformation, polymerase chain reaction (PCR), gel electrophoresis, and the use of several bioinformatics tools. These laboratory exercises would be conducted as a mini research project in which all the classes would be connected with the previous ones. This curriculum was compared in an experiment involving two groups of students from two different majors. The new curriculum, with classes linked together as a mini research project, was taught to a major in Pharmacy and an old curriculum was taught to students from environmental health. The results showed that students who were enrolled in the new curriculum obtained better results in the final exam than the students who were enrolled in the former curriculum. Likewise, these students were found to be more enthusiastic during the laboratory classes than those from the former curriculum.por
dc.identifier.doi10.1002/bmb.20324pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/3602
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relation.ispartofseriesBiochemistry and Molecular Biology Education; Vol. 37, Nº 5
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/bmb.20324/abstractpor
dc.subjectCurriculum developmentpor
dc.subjectGenomics proteomics bioinformaticspor
dc.subjectLaboratory exercisespor
dc.subjectRecombinant DNA technologypor
dc.subjectIntegration of research into undergraduate teachingpor
dc.titleß-lactamases in the biochemistry and molecular biology laboratorypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage306por
oaire.citation.issueNº 5por
oaire.citation.startPage301por
oaire.citation.titleBiochemistry and Molecular Biology Educationpor
oaire.citation.volumeVol. 37por
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person.givenNameCristina
person.givenNameMónica
person.givenNameRicardo
person.givenNamePedro
person.givenNameRúben
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rcaap.rightsopenAccesspor
rcaap.typearticlepor
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