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Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction

2012Journal article Open access
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dc.contributor.authorRamalhosa, Maria João
dc.contributor.authorPaíga, Paula
dc.contributor.authorMorais, Simone
dc.contributor.authorSousa, Ana M. M.
dc.contributor.authorGonçalves, Maria Pilar
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorOliveira, M. Beatriz P. P.
dc.date.accessioned2013-09-30T11:02:04Z
dc.date.available2013-09-30T11:02:04Z
dc.date.issued2012
dc.description.abstractAn accurate and sensitive method for determination of 18 polycyclic aromatic hydrocarbons (PAHs) (16 PAHs considered by USEPA as priority pollutants, dibenzo[a,l]pyrene and benzo[j]fluoranthene) in fish samples was validated. Analysis was performed by microwave-assisted extraction and liquid chromatography with photodiode array and fluorescence detection. Response surface methodology was used to find the optimal extraction parameters. Validation of the overall methodology was performed by spiking assays at four levels and using SRM 2977. Quantification limits ranging from 0.15–27.16 ng/g wet weight were obtained. The established method was applied in edible tissues of three commonly consumed and commercially valuable fish species (sardine, chub mackerel and horse mackerel) originated from Atlantic Ocean. Variable levels of naphthalene (1.03–2.95 ng/g wet weight), fluorene (0.34–1.09 ng/g wet weight) and phenanthrene (0.34–3.54 ng/g wet weight) were detected in the analysed samples. None of the samples contained detectable amounts of benzo[a]pyrene, the marker used for evaluating the occurrence and carcinogenic effects of PAHs in food.por
dc.identifier.doi10.101/j.foodchem.2012.04.078pt_PT
dc.identifier.doi10.101/j.foodchem.2012.04.078pt_PT
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10400.22/2037
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofseriesFood Chemistry; Vol. 135, Issue 1
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0308814612007418por
dc.subjectPolycyclic aromatic hydrocarbonspor
dc.subjectFishpor
dc.subjectMicrowave-assisted extractionpor
dc.subjectResponse surface methodologypor
dc.subjectLiquid chromatographypor
dc.titleAnalysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extractionpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage242por
oaire.citation.issueIssue 1
oaire.citation.startPage234por
oaire.citation.volumeVol. 135por
person.familyNameRamalhosa
person.familyNamePaíga
person.familyNameMorais
person.familyNameDelerue-Matos
person.givenNameMaria João
person.givenNamePaula
person.givenNameSimone
person.givenNameCristina
person.identifier1668889
person.identifier1598822
person.identifier.ciencia-id421A-C491-F45B
person.identifier.ciencia-id331A-5D6A-D3C8
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-1951-3557
person.identifier.orcid0000-0002-9593-1355
person.identifier.orcid0000-0001-6433-5801
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-8293-2013
person.identifier.ridQ-4930-2018
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id35488836000
person.identifier.scopus-author-id6506638953
person.identifier.scopus-author-id7007053747
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscovery7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9

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