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Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction
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Orientador(es)
Resumo(s)
An accurate and sensitive method for determination of 18 polycyclic aromatic hydrocarbons (PAHs) (16
PAHs considered by USEPA as priority pollutants, dibenzo[a,l]pyrene and benzo[j]fluoranthene) in fish
samples was validated. Analysis was performed by microwave-assisted extraction and liquid chromatography
with photodiode array and fluorescence detection. Response surface methodology was used to find
the optimal extraction parameters. Validation of the overall methodology was performed by spiking
assays at four levels and using SRM 2977. Quantification limits ranging from 0.15–27.16 ng/g wet weight
were obtained. The established method was applied in edible tissues of three commonly consumed and
commercially valuable fish species (sardine, chub mackerel and horse mackerel) originated from Atlantic
Ocean. Variable levels of naphthalene (1.03–2.95 ng/g wet weight), fluorene (0.34–1.09 ng/g wet weight)
and phenanthrene (0.34–3.54 ng/g wet weight) were detected in the analysed samples. None of the samples
contained detectable amounts of benzo[a]pyrene, the marker used for evaluating the occurrence and
carcinogenic effects of PAHs in food.
Descrição
Palavras-chave
Polycyclic aromatic hydrocarbons Fish Microwave-assisted extraction Response surface methodology Liquid chromatography
Contexto Educativo
Citação
Editora
Elsevier