Logo do repositório
 
Publicação

Optimization of SPE-HPLC-FLD methodologies for the extraction and detection of steroid hormones in plasma

2026-05-29Documento de conferência Acesso aberto
Ver registo simples
dc.contributor.authorBracchi, Isabella
dc.contributor.authorPaíga, Paula
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorPestana, Diogo
dc.contributor.authorNegrão, Rita
dc.contributor.authorKeating, Elisa
dc.contributor.authorFernandes, Virgínia
dc.date.accessioned2026-07-28T09:15:35Z
dc.date.available2026-07-28T09:15:35Z
dc.date.issued2026-05-29
dc.descriptionNORTE-01-0145-FEDER-000039
dc.description.abstractThe quantification of steroid hormones, such as es-tradiol and ethinylestradiol, in plasma requires sensi-tive and reliable analytical methodologies1. This study presents the optimization of a bioanalytical method based on Solid-Phase Extraction (SPE) coupled with High-Performance Liquid Chromatography with Flu-orescence Detection (HPLC-FLD). For sample ex-traction, Strata-X (polymeric reversed-phase) cartridg-es were used, where critical SPE parameters, including cartridge conditioning, matrix washing, and analyte elution, were evaluated. Chromatographic separation was achieved on a C18 stationary phase using a mobile phase of acetonitrile and 0.1% formic acid in ultrapure water. Both isocratic and gradient elution programs were tested to maximize resolution and peak shape. The method was validated for linearity, recovery, and precision (intra- and inter-day), meeting all established criteria for bioanalytical applications. This combined optimization resulted in improved selectivity and sen-sitivity for the determination of estradiol and ethinyl-estradiol in plasma (Figure 1), demonstrating a robust bioanalytical tool for monitoring these compounds in complex biological matrices.eng
dc.description.sponsorshipNORTE-01-0145-FEDER-000039
dc.identifier.citationBracchi, I., Paíga, P., Delerue-Matos, C., Pestana, D., Negrão, R., Keating, E., & Fernandes, V. (2026). Optimization of SPE-HPLC-FLD methodologies for the extraction and detection of steroid hormones in plasma. Book of Abstracts of the 8th Meeting on Medicinal Biotechnology, 29. https://edicoes.ipp.pt/index.php/books/catalog/book/251
dc.identifier.doidoi.org/10.26537/ed.p.porto.251
dc.identifier.isbn978-989-9226-20-3
dc.identifier.urihttp://hdl.handle.net/10400.22/32635
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPolitema
dc.relation2023.03003.BD; UID/50006/2025
dc.relation.hasversionhttps://edicoes.ipp.pt/index.php/books/catalog/book/251
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectHigh-performance liquid chromatography
dc.subjectSteroid hormones
dc.subjectSolid-phase extraction
dc.titleOptimization of SPE-HPLC-FLD methodologies for the extraction and detection of steroid hormones in plasmaeng
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferenceDate2026-05-29
oaire.citation.conferencePlacePorto
oaire.citation.endPage29
oaire.citation.startPage29
oaire.citation.titleBook of Abstracts of the 8th Meeting on Medicinal Biotechnology
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
POSTER_Isabella Bracchi.pdf
Tamanho:
1.22 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
4.03 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

ESS - CF - Posters apresentados em eventos científicos
ESS - CQB - Posters apresentados em eventos científicos
ESS - TBIO/Rise Health - Posters apresentados em eventos científicos