Repository logo
 
Publication

Phytochemical Characterization and Biological Evaluation of the Aqueous and Supercritical Fluid Extracts from Salvia sclareoides Brot

2017Journal article Open access
Simple item page
dc.contributor.authorBatista, Daniela
dc.contributor.authorFalé, Pedro L.
dc.contributor.authorSerralheiro, Maria L.
dc.contributor.authorAraújo, Maria-Eduarda
dc.contributor.authorDias, Catarina
dc.contributor.authorBranco, Isabel
dc.contributor.authorGrosso, Clara
dc.contributor.authorCoelho, José
dc.contributor.authorPalavra, António
dc.contributor.authorMadeira, Paulo J. A.
dc.contributor.authorMartins, Alice
dc.contributor.authorRauter, Amélia P.
dc.date.accessioned2019-06-06T09:16:26Z
dc.date.available2019-06-06T09:16:26Z
dc.date.issued2017
dc.description.abstractPlants belonging to the genus Salvia (Lamiaceae) are known to have a wide range of biological properties. In this work, extracts obtained from the aerial parts of Salvia sclareoides Brot. were evaluated to investigate their chemical composition, toxicity, bioactivity, and stability under in vitro gastrointestinal conditions. The composition of the supercritical fluid extract was determined by GC and GC-MS, while the identification of the infusion constituents was performed by HPLC-DAD and LC-MS. The in vitro cytotoxicity of both extracts (0-2 mg/mL) was evaluated in Caco-2 cell lines by the MTT assay. The anti-inflammatory and anticholinesterase activities were determined through the inhibition of cyclooxygenase-1 and acetylcholinesterase enzymes, while β-carotene/linoleic acid bleaching test and the DPPH assays were used to evaluate the antioxidant activity. The infusion inhibited cyclooxygenase-1 (IC50 = 271.0 μg/mL), and acetylcholinesterase (IC50 = 487.7 μg/ mL) enzymes, also demonstrated significant antioxidant properties, as evaluated by the DPPH (IC50 = 10.4 μg/mL) and β-carotene/linoleic acid (IC50 = 30.0 μg/mL) assays. No remarkable alterations in the composition or in the bioactivities of the infusion were observed after in vitro digestion, which supports the potential of S. sclareoides as a source of bioactive ingredients with neuroprotective, anti-inflammatory and antioxidant properties.pt_PT
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e a Tecnologia (FCT), Project UID/MULTI/00612/2013 and UID/QUI/50006/2013. FCT is also acknowledged for PJAM post-doctoral fellowship (SFRH/BPD/86948/2012) and for CG FCT Investigator (IF/01332/2014). The European Commission is also acknowledged for approval of the INOVAFUNAGEING commitment and the support of the projects PERSSILAA-FP7-ICT-2013-10, Project Nr. 610359, and D3i4AD, FP7-PEOPLE-2013-IAPP, GA 612347. The authors also gratefully acknowledge the collaboration of Prof. Ana Cristina Figueiredo (DBV-FCUL), for GC and GC-MS analysis, and of Prof. Maria Helena Florêncio (DQB-FCUL) for LC-MS support.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1515/chem-2017-0011pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/13855
dc.language.isoengpt_PT
dc.publisherDe Gruyterpt_PT
dc.relationA MASS SPECTROMETRY APPROACH TO UNRAVEL THE METABOLIC PATHWAYS OF ANTIMALARIAL HYBRID COMPOUNDS
dc.relation.publisherversionhttps://www.degruyter.com/view/j/chem.2017.15.issue-1/chem-2017-0011/chem-2017-0011.xmlpt_PT
dc.subjectSalvia sclareoidespt_PT
dc.subjectGC-MSpt_PT
dc.subjectHPLCpt_PT
dc.subjectBioactivitypt_PT
dc.subjectIn vitro digestionpt_PT
dc.titlePhytochemical Characterization and Biological Evaluation of the Aqueous and Supercritical Fluid Extracts from Salvia sclareoides Brotpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleA MASS SPECTROMETRY APPROACH TO UNRAVEL THE METABOLIC PATHWAYS OF ANTIMALARIAL HYBRID COMPOUNDS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00612%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F86948%2F2012/PT
oaire.citation.endPage91pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage82pt_PT
oaire.citation.titleOpen Chemistrypt_PT
oaire.citation.volume15pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameGrosso
person.givenNameAna Clara
person.identifier.ciencia-id711B-1170-4C32
person.identifier.orcid0000-0002-2457-8267
person.identifier.ridB-9813-2013
person.identifier.scopus-author-id25627935200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf8a4443b-2748-4523-8219-691d0523d467
relation.isAuthorOfPublication.latestForDiscoveryf8a4443b-2748-4523-8219-691d0523d467
relation.isProjectOfPublication16cdf8bf-19df-45d6-88a3-f664c21813af
relation.isProjectOfPublication5c06a623-3499-408c-aef9-0a1f68cb1d02
relation.isProjectOfPublicationf3acb791-04bd-45dc-9726-da684109ea33
relation.isProjectOfPublication.latestForDiscovery16cdf8bf-19df-45d6-88a3-f664c21813af

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ART_DanielaBatista_2017_1.pdf
Size:
1.12 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEP – GRAQ – Artigos