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Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese

dc.contributor.authorAmador, Paula
dc.contributor.authorFernandes, Rúben
dc.contributor.authorPrudêncio, Cristina
dc.contributor.authorBrito, Luísa
dc.date.accessioned2013-09-20T14:19:57Z
dc.date.available2013-09-20T14:19:57Z
dc.date.issued2009
dc.description.abstractThe purpose of this study was to investigate the presence of beta-lactam-resistant bacteria in six different types of Portuguese cheese. The numbers of ampicillin resistant (AMP(r)) bacteria varied from 4.7 x 10(2) to 1.5 x 10(7) CFU/g. Within 172 randomly selected beta-lactam-resistant bacteria, 44 resistant phenotypes were found and 31.4% were multidrug resistant. The majority (85%) of the isolates identified belonged to the Enterobacteriaceae family. The presence of the bla(TEM) gene was detected in 80.9% of the tested isolates. The results suggest that without thermal processing of the milk and good hygienic practices, cheese may act as a vehicle of transfer of beta-lactam-resistant bacteria to the gastrointestinal tract of consumers.por
dc.identifier.doi10.3390/ijms10041538pt_PT
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.22/1982
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherMDPIpor
dc.relation.publisherversionhttp://www.mdpi.com/1422-0067/10/4/1538por
dc.subjectReady-to-eat (RTE) foodpor
dc.subjectCheese, minimal inhibitory concentration (MIC)por
dc.subjectβ-lactam-resistant enterobacteriaceaepor
dc.subjectMultiplex-PCRpor
dc.subjectBlaTEM genepor
dc.titleResistance to β-lactams in bacteria isolated from different types of Portuguese cheesepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1551por
oaire.citation.startPage1538por
oaire.citation.titleInternational Journal of Molecular Sciencespor
person.familyNameFernandes
person.familyNamePrudêncio
person.givenNameRúben
person.givenNameCristina
person.identifier635792
person.identifier1200571
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.orcid0000-0001-8933-3984
person.identifier.orcid0000-0002-9920-936X
person.identifier.scopus-author-id57640135700
person.identifier.scopus-author-id6508057930
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication.latestForDiscovery881a8ad5-ab13-4e49-89f4-08ca61cc81e3

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