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Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum

2018Journal article Open access
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dc.contributor.authorOliveira, Ana Isabel
dc.contributor.authorPinho, Cláudia
dc.contributor.authorFonte, Pedro
dc.contributor.authorSarmento, Bruno
dc.contributor.authorDias, Alberto C.P.
dc.date.accessioned2019-06-05T15:40:02Z
dc.date.available2019-06-05T15:40:02Z
dc.date.issued2018
dc.description.abstractQuercetin and biapigenin are antioxidant and neuroprotective compounds present in Hypericum perforatum, with potential application in neurodegenerative diseases. Quercetin has shown to have excellent antioxidant activities. Biapigenin possesses a distinctive mechanism of action, preventing the onset of calcium deregulation and mitochondrial dysfunction. The main aim of this study was to establish a new delivery system encapsulating H. perforatum neuroprotective fraction, isolated from it,containing quercetin and biapigenin into poly(Ɛ-caprolactone) (PCL) nanoparticles. It also aimed to study its hepatoprotective potential. Different formulation parameters were optimized, resulting in a stable formulation of polymer:compounds ratio 1:0.1 with a mean particle size of 185nm, zeta potential around -20mV and association efficiency close to 100%. Compounds in vitro release, under physiological conditions, showed an initial burst followed by a sustained release. The antioxidant functional properties of these compounds were not altered by encapsulation. PCL-loaded nanoparticles protected HepG2 cells from intrinsic compound toxicity at high concentrations. Depending on the incubation regimen, quercetin-biapigenin PCL-loaded nanoparticles or free compounds were more effective in protecting HepG2 cells against tert-butylhydroperoxide-induced toxicity. This is the first report of the encapsulation of a quercetin-biapigenin mixture in a polymeric matrix, specifically, in PCL, with synergic anti-oxidant and hepatoprotective effects.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ijbiomac.2017.10.103pt_PT
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/10400.22/13848
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPTDC/AGR-ALI/105169/2008, PEst-OE/AGR/UI4033/2014pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0141813017320512pt_PT
dc.subjectHep G2 Cellspt_PT
dc.subjectHumanspt_PT
dc.subjectHypericumpt_PT
dc.subjectAntioxidantspt_PT
dc.subjectDrug Carrierspt_PT
dc.subjectNanoparticlespt_PT
dc.subjectNeuroprotective Agentspt_PT
dc.subjectPolyesterspt_PT
dc.titleDevelopment, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage196pt_PT
oaire.citation.startPage185pt_PT
oaire.citation.titleInternational Journal of Biological Macromoleculespt_PT
oaire.citation.volume110pt_PT
person.familyNameOliveira
person.familyNamePinho
person.givenNameAna Isabel
person.givenNameCláudia
person.identifier.ciencia-id8B1B-A0D9-B6E4
person.identifier.orcid0000-0002-3160-6596
person.identifier.orcid0000-0002-3157-3712
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb38c382c-5355-4b8e-aae0-45af3aa1dc8e
relation.isAuthorOfPublication01c994b8-cb20-43a8-947c-d2b978283944
relation.isAuthorOfPublication.latestForDiscoveryb38c382c-5355-4b8e-aae0-45af3aa1dc8e

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