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Electrochemical sensing of ecstasy with electropolymerized molecularly imprinted poly(o-phenylenediamine) polymer on the surface of disposable screen-printed carbon electrodes

2019-03Journal article Open access
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dc.contributor.authorCouto, Rosa A.S.
dc.contributor.authorCosta, Séfora S.
dc.contributor.authorMounssef, Bassim
dc.contributor.authorPacheco, João
dc.contributor.authorFernandes, Eduarda
dc.contributor.authorCarvalho, Félix
dc.contributor.authorRodrigues, Cecília M.P.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorBraga, Ataualpa A.C.
dc.contributor.authorMoreira Gonçalves, Luís
dc.contributor.authorQuinaz, M. Beatriz
dc.date.accessioned2020-06-25T13:12:40Z
dc.date.available2020-06-25T13:12:40Z
dc.date.issued2019-03
dc.description.abstractThis study demonstrates the ability of an electrochemical sensor based on molecularly imprinted polymers (MIPs) to selectively quantify 3,4-methylenedioxymethamphetamine (MDMA), also known as ecstasy, in biological samples. The device was constructed using ortho-phenylenediamine (o-PD) as the MIP’s building monomer at the surface of a screen-printed carbon electrode (SPCE). The step-by-step construction of the SPCE-MIP sensor was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Density functional theory (DFT) calculations and modelling were performed not only to understand template-monomer interaction but also to comprehend which possible polymer structure - linear or ramified poly(o-PD) – indeed interacts with the analyte. The prepared sensor worked by directly measuring the MDMA oxidation signal through square-wave voltammetry (SWV) after an incubation period of 10 min. Several parameters were optimized, such as the monomer/template ratio, the number of electropolymerization scanning cycles, and the incubation period, to obtain the best sensing efficiency. Optimized sensors exhibited suitable selectivity, repeatability (2.6%), reproducibility (7.7%) and up to one month of stable response. A linear range up to 0.2 mmol L−1 was found with an r2 of 0.9990 and a limit of detection (LOD) and quantification (LOQ) of 0.79 and 2.6 μmol L−1 (0.15 and 0.51 μg mL−1), respectively. The proposed sensor was successfully applied to human blood serum and urine samples, showing its potential for application in medicine and in forensic sciences.pt_PT
dc.description.sponsorshipThis work received financial support from FCT/MCTES through national funds and was co-financed by FEDER, under Partnership Agreement PT2020-UID/QUI/50006/2013-POCI/01/0145/FEDER/007265. RASC wishes to acknowledge FCT for her PhD fellowship (PD/BD/127797/2016) from the PhD Programme in Medicines and Pharmaceutical Innovation (i3DU). AACB (grants 2014/25770-6 and 2015/01491-3) and LMG (grant 2018/14425-7) thank the São Paulo Research Foundation (FAPESP) for financial support. AACB (grant 309715/2017-2) also thanks the Brazilian National Research Council (CNPq) for financial support and fellowships. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2019.03.138pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16008
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0925400519305040?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAmphetaminespt_PT
dc.subjectComputational modellingpt_PT
dc.subjectElectroanalysispt_PT
dc.subjectModified working electrodept_PT
dc.subjectMolecular recognitionpt_PT
dc.subjectNon-labelled sensing of recreational drugspt_PT
dc.titleElectrochemical sensing of ecstasy with electropolymerized molecularly imprinted poly(o-phenylenediamine) polymer on the surface of disposable screen-printed carbon electrodespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage386pt_PT
oaire.citation.startPage378pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume290pt_PT
person.familyNamePacheco
person.familyNameDelerue-Matos
person.givenNameJoão
person.givenNameCristina
person.identifier.ciencia-id8D13-F178-F44D
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-8366-0490
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id35519962400
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd281363a-9eb0-47b4-b8ba-1a98bacd8d09
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscoveryd281363a-9eb0-47b4-b8ba-1a98bacd8d09

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