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Unveiling the contribution of ars operon for arsenic tolerance among clinically-relevant Salmonella serotypes/clones

2017-12Conference object Open access
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dc.contributor.authorMourão, Joana
dc.contributor.authorRebelo, Andreia
dc.contributor.authorMachado, Jorge
dc.contributor.authorAlmeida, Agostinho
dc.contributor.authorPeixe, Luísa
dc.contributor.authorNovais, Carla
dc.contributor.authorAntunes, Patrícia
dc.date.accessioned2024-12-18T09:34:23Z
dc.date.available2024-12-18T09:34:23Z
dc.date.issued2017-12
dc.description.abstractArsenic environmental contamination by anthropogenic activities, including in animal-farming management (e.g. coccidiostatics/pesticides/waste), may represent a long-term selective pressure driving the selection of multidrug-resistant (MDR) emergent Salmonella serotypes/clones. Diverse arsenic tolerance (AsT) mechanisms were described, although dispersion and association with a tolerance phenotype remains unknown in Salmonella. Our objective was to study the occurrence of genes coding for arsenical efflux pumps and their implications in tolerance phenotypes in diverse Salmonella serotypes and clones, including emergent MDR and/or copper/silver tolerant ones. 283 Salmonella isolates (2000-2016; humans/foods/animal/environment) from 57 serotypes (including the most frequent: Enteritidis/n=16; Typhimurium/n=49; 4,[5],12:i:-/n=63; Rissen/n=15) were selected based in PFGE-types and/or STs obtained by MLST and different profiles of antibiotic-resistance or metal-tolerance (Cu/Ag/Hg/Te)1,2. Screening of arsenic tolerance genes, arsB and acr3 (both coding for arsenical pump membrane proteins) was performed by PCR/sequencing. MICNa2HAsO4 were determined in aerobic and anaerobic atmospheres by the agar dilution method.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMourão, J., Rebelo, A., Mahado, J., Almeida, A., Peixe, Luísa, Novais, C., & Antunes, P. (2017). Unveiling the contribution of ars operon for arsenic tolerance among clinically-relevant Salmonella serotypes/clones. Book of abstracts - Congress of Microbiology and Biotechnology 2017 - MICROBIOTEC 17, 148.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/26904
dc.language.isoengpt_PT
dc.publisherUniversidade Católica/Sociedade Portuguesa de Microbiologia/Sociedade Portuguesa de Biotecnologiapt_PT
dc.relationThis work was supported by FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 [grant UID/MULTI/04378/2013 – POCI/01/0145/FERDER/007728]; the project NORTE-01-0145-FEDER- 000011; Joana Mourão has a Post-doc fellowship supported by project NORTE-01-0145-FEDER-000024 and Andreia Rebelo a MSc fellowship supported by project UID/QUI/50006. The authors are greatly indebted to all of the financing sources.pt_PT
dc.relation.publisherversionhttps://www.porto.ucp.pt/sites/default/files/files/Biotecnologia/Microbiotec17/BookofAbstracts_15_12_2017.pdfpt_PT
dc.subjectSalmonellapt_PT
dc.subjectArsenicpt_PT
dc.subjectMultidrug-resistancept_PT
dc.subjectMetal-tolerancept_PT
dc.subjectPig-associated clonespt_PT
dc.titleUnveiling the contribution of ars operon for arsenic tolerance among clinically-relevant Salmonella serotypes/clonespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortopt_PT
oaire.citation.startPage148pt_PT
oaire.citation.titleBook of abstracts - Congress of Microbiology and Biotechnology 2017 - MICROBIOTEC 17pt_PT
person.familyNameRebelo
person.givenNameAndreia
person.identifier.ciencia-idA81C-7A59-ECAD
person.identifier.orcid0000-0003-2218-6005
person.identifier.ridT-9024-2017
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication913dc5cd-9e92-456d-88f5-419571a87f85
relation.isAuthorOfPublication.latestForDiscovery913dc5cd-9e92-456d-88f5-419571a87f85

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