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Advisor(s)
Abstract(s)
Arsenic environmental contamination by anthropogenic activities, including in animal-farming management (e.g. coccidiostatics/pesticides/waste), may represent a long-term selective pressure driving the selection of multidrug-resistant (MDR) emergent Salmonella serotypes/clones. Diverse arsenic tolerance (AsT) mechanisms were described, although dispersion and association with a tolerance phenotype remains unknown in Salmonella. Our objective was to study the occurrence of genes coding for arsenical efflux pumps and their implications in tolerance phenotypes in diverse Salmonella serotypes and clones, including emergent MDR and/or copper/silver tolerant ones. 283 Salmonella isolates (2000-2016; humans/foods/animal/environment) from 57 serotypes (including the most frequent: Enteritidis/n=16; Typhimurium/n=49; 4,[5],12:i:-/n=63; Rissen/n=15) were selected based in PFGE-types and/or STs obtained by MLST and different profiles of antibiotic-resistance or metal-tolerance (Cu/Ag/Hg/Te)1,2. Screening of arsenic tolerance genes, arsB and acr3 (both coding for arsenical pump membrane proteins) was performed by PCR/sequencing. MICNa2HAsO4 were determined in aerobic and anaerobic atmospheres by the agar dilution method.
Description
Keywords
Salmonella Arsenic Multidrug-resistance Metal-tolerance Pig-associated clones
Pedagogical Context
Citation
Mourão, J., Rebelo, A., Mahado, J., Almeida, A., Peixe, Luísa, Novais, C., & Antunes, P. (2017). Unveiling the contribution of ars operon for arsenic tolerance among clinically-relevant Salmonella serotypes/clones. Book of abstracts - Congress of Microbiology and Biotechnology 2017 - MICROBIOTEC 17, 148.
Publisher
Universidade Católica/Sociedade Portuguesa de Microbiologia/Sociedade Portuguesa de Biotecnologia