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Cytotoxicity of marine cyanobacteria extracts on osteosarcoma cells

2012-05Conference object Open access
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dc.contributor.authorCosta-Rodrigues, João
dc.contributor.authorCosta, M. M.
dc.contributor.authorCosta, S.
dc.contributor.authorGarcia, M.
dc.contributor.authorFernandes, M. H.
dc.contributor.authorVasconcelos, V.
dc.contributor.authorBarros, Piedade
dc.contributor.authorMartins, Rosário
dc.date.accessioned2021-09-28T14:56:10Z
dc.date.available2021-09-28T14:56:10Z
dc.date.issued2012-05
dc.description.abstractMarine cyanobacteria have been identified as a rich source of secondary metabolites with potential pharmacological applications. Anti-inflammatory, antibacterial and anticancer activities are some examples of properties described for cyanobacteria compounds, being the cytoxicity against cancer cell lines one of the most documented. The Laboratory of Ecotoxicology Genomics and Evolution (LEGE) — CIIMAR, Porto, Portugal, possesses a large collection of cyanobacteria strains isolated from the Portuguese coast. In order to investigate the interest of genera such as Cyanobium, Synechocystis, Synechococcus, Leptolyngbya and Pseudoanabaena which are genera that have been largely overlooked in terms of bioactivity, we have been screening their ability to induce cytotoxicity on human cancer cell lines. Assays have been conducted with a crude extract obtained by a dichloromethane and methanol extraction of freeze dried biomass and three fractions obtained using Si column chromatography with a gradient from 100% hexane, to 100% ethyl acetate to 100% methanol. The cytotoxicity of cyanobacteria crude extract and fractions has been evaluated by the MTT assay at 24, 48 and 72 h. Here we present the results concerning the cytotoxicity of 24 cyanobacteria strains on the osteosarcoma cell line MG63. The results show both inhibitory and stimulatory effects on cell growth within the same cyanobacteria strain. However, five cyanobacteria strains were found to induce a decrease in cell viability that reached the 80% within the ethyl acetate fraction, which makes this fraction interesting for the isolation and characterization of new bioactive compounds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta-Rodrigues, J., Costa, M., Costa, S., Garcia, M., Fernandes, M. H., Vasconcelos, V., Barros, P., & Martins, R. (2012). Cytotoxicity of marine cyanobacteria extracts on osteosarcoma cells. Bone, 50, S181-S182. https://doi.org/https://doi.org/10.1016/j.bone.2012.02.574pt_PT
dc.identifier.doi10.1016/j.bone.2012.02.574pt_PT
dc.identifier.issn8756-3282
dc.identifier.urihttp://hdl.handle.net/10400.22/18608
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S8756328212006461?via%3Dihub#!pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCyanobacteriapt_PT
dc.subjectCytoxicitypt_PT
dc.titleCytotoxicity of marine cyanobacteria extracts on osteosarcoma cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceStockholm, SWEDENpt_PT
oaire.citation.endPage182pt_PT
oaire.citation.startPage181pt_PT
oaire.citation.title39th Annual Congress of the European-Calcified-Tissue-Society (ECTS)pt_PT
oaire.citation.volume50pt_PT
person.familyNameCosta-Rodrigues
person.familyNameGonçalves de Barros
person.familyNameMartins
person.givenNameJoao
person.givenNamePiedade Aurora
person.givenNameRosario
person.identifier.ciencia-id7412-B006-2120
person.identifier.ciencia-idD210-6003-454B
person.identifier.ciencia-idA919-DB40-EF10
person.identifier.orcid0000-0003-1375-8067
person.identifier.orcid0000-0002-2635-251X
person.identifier.orcid0000-0002-9882-4651
person.identifier.scopus-author-id8558383400
person.identifier.scopus-author-id23981959400
person.identifier.scopus-author-id21834368700
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationce85cc8f-09f3-4c26-aeaf-6128971acf68
relation.isAuthorOfPublicationa85b368a-8f13-45eb-a744-7c5810a38e2c
relation.isAuthorOfPublication82a7c349-c88e-450f-adc7-3331d69149cf
relation.isAuthorOfPublication.latestForDiscoveryce85cc8f-09f3-4c26-aeaf-6128971acf68

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