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Lycopene-rich extract from red guava ( Psidium guajava L.) displays cytotoxic effect against human breast adenocarcinoma cell line MCF-7 via an apoptotic-like pathway

2018Journal article Open access
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dc.contributor.authorSantos, Raimunda C. dos
dc.contributor.authorOmbredane, Alicia S.
dc.contributor.authorSouza, Jéssica Maria T.
dc.contributor.authorVasconcelos, Andreanne G.
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorAmorim, Adriany das G.N.
dc.contributor.authorBarbosa, Eder Alves
dc.contributor.authorLima, Filipe C.D.A.
dc.contributor.authorRopke, Cristina D.
dc.contributor.authorAlves, Michel M.M.
dc.contributor.authorArcanjo, Daniel D.R.
dc.contributor.authorCarvalho, Fernando A.A.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorJoanitti, Graziella A.
dc.contributor.authorLeite, José Roberto de S.A.
dc.date.accessioned2019-10-07T13:58:17Z
dc.date.available2019-10-07T13:58:17Z
dc.date.issued2018
dc.description.abstractThis study investigated a lycopene-rich extract from red guava (LEG) for its chemical composition using spectrophotometry, mass spectrometry, attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), and computational studies. The cytotoxic activity of LEG and the underlying mechanism was studied in human breast adenocarcinoma cells (MCF-7), murine fibroblast cells (NIH-3T3), BALB/c murine peritoneal macrophages, and sheep blood erythrocytes by evaluating the cell viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and flow cytometry. Spectrophotometry analysis showed that LEG contained 20% of lycopene per extract dry weight. Experimental and theoretical ATR-FTIR suggests the presence of lycopene, whereas MS/MS spectra obtained after fragmentation of the molecular ion [M]+• of 536.4364 show fragment ions at m/z 269.2259, 375.3034, 444.3788, and 467.3658, corroborating the presence of lycopene mostly related to all-trans configuration. Treatment with LEG (1600 to 6.25μg/mL) for 24 and 72h significantly affected the viability of MCF-7 cells (mean half maximal inhibitory concentration [IC50]=29.85 and 5.964μg/mL, respectively) but not NIH-3T3 cells (IC50=1579 and 911.5μg/mL, respectively). Furthermore LEG at concentrations from 800 to 6.25μg/mL presented low cytotoxicity against BALB/c peritoneal macrophages (IC50≥800μg/mL) and no hemolytic activity. LEG (400 and 800μg/mL) caused reduction in the cell proliferation and induced cell cycle arrest, DNA fragmentation, modifications in the mitochondrial membrane potential, and morphologic changes related to granularity and size in MCF-7 cells; however, it failed to cause any significant damage to the cell membrane or display necrosis or traditional apoptosis. In conclusion, LEG was able to induce cytostatic and cytotoxic effects on breast cancer cells probably via induction of an apoptotic-like pathway.pt_PT
dc.description.sponsorshipThe authors acknowledge the computational time provided by CENAPAD/SP on the project proj697. Alexandra Plácido is gratefully to FCT by her grant SFRH/BD/97995/2013, financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. The work at REQUIMTE/LAQV received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT) through project UID/QUI/50006/2013. Adriany das G. N. Amorim is grateful to CAPES by for the doctoral fellowship process no. 99999.004236/2014-09 in Federal University of Piauí (UFPI). Eder A. Barbosa is grateful to PNPD/CAPES for its post-doctoral fellowship.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.foodres.2017.10.045
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10400.22/14707
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0963996917307305?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCarotenoidpt_PT
dc.subjectAntioxidantpt_PT
dc.subjectBreast cancerpt_PT
dc.subjectCytotoxicitypt_PT
dc.titleLycopene-rich extract from red guava ( Psidium guajava L.) displays cytotoxic effect against human breast adenocarcinoma cell line MCF-7 via an apoptotic-like pathwaypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97995%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.endPage196pt_PT
oaire.citation.startPage184pt_PT
oaire.citation.titleFood Research Internationalpt_PT
oaire.citation.volume105pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream5876
person.familyNamePlácido
person.familyNameDelerue-Matos
person.givenNameAlexandra
person.givenNameCristina
person.identifier1621053
person.identifier.ciencia-id1B1A-461B-E8DD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0003-2706-7777
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridL-3085-2014
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id55232677900
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationaad1737a-79f4-4c05-a480-3c0197877757
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
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relation.isProjectOfPublicationdf750fb0-a21e-43b2-8a67-f8607fefefcf
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