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Adipocyte proteome and secretome influence inflammatory and hormone pathways in glioma

2018Journal article Open access
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dc.contributor.authorAlmeida, Joana
dc.contributor.authorCosta, J.
dc.contributor.authorCoelho, Pedro
dc.contributor.authorCea, V.
dc.contributor.authorGalesio, M.
dc.contributor.authorNoronha, J. P.
dc.contributor.authorDiniz, M. S.
dc.contributor.authorPrudêncio, Cristina
dc.contributor.authorSoares, R.
dc.contributor.authorSala, C.
dc.contributor.authorFernandes, Rúben
dc.date.accessioned2019-07-01T17:09:23Z
dc.date.available2020-05-02T00:30:30Z
dc.date.issued2018
dc.description.abstractGliomas represent the most common primary malignant brain tumors in adults, with an extremely poor prognosis. Among several risk factors, lifestyle was also recently identified as a major risk factor for the development of primary glioma. In the present study, we explore the relationship between obesity and glioma in a cellular model. Thus, we have study the influence of adipocytes secretome on glioma cell line GL261. Using the 3T3-L1 adipocyte cell line, and its conditioned medium (adipokines-enriched medium), we showed that adipocyte-released factors relate with glioma angiogenic, growth, hormones and metabolic behavior by MALDI-TOF-MS and proteomic array analysis. In a first view, STI1, hnRNPs and PGK1 are under expressed on CGl. Similarly, both carbonic anhydrase and aldose reductase are even suppressed in glioma cells that grown under adipokines-enriched environment. Contrariwise, RFC1, KIF5C, ANXA2, N-RAP and RACK1 are overexpressed in GL261 cell the in the presence of the adipokines-enriched medium. We further identified the factors that are released by adipocyte cells, and revealed that several pro-inflammatory and angiogenic factors, such as IL-6, IL-11, LIF, PAI-1, TNF-α, endocan, HGF, VEGF IGF-I, were secreted to the medium into a high extent, whereas TIMP-1 and SerpinE1 were under expressed on CGl. This study discloses an interesting in vitro model for the study of glioma biology under a "obesity" environment, that can be explored for the understanding of cancer cells biology, for the search of biomarkers, prognostic markers and therapeutic approaches.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlmeida, J., Costa, J., Coelho, P., Cea, V., Galesio, M., Noronha, J. P., Diniz, M. S., Prudêncio, C., Soares, R., Sala, C., & Fernandes, R. (2019). Adipocyte proteome and secretome influence inflammatory and hormone pathways in glioma. Metabolic Brain Disease, 34(1), 141–152. https://doi.org/10.1007/s11011-018-0327-y
dc.identifier.doi10.1007/s11011-018-0327-ypt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14213
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s11011-018-0327-ypt_PT
dc.subject3T3-L1 Cellspt_PT
dc.subjectAdipocytespt_PT
dc.subjectAnimalspt_PT
dc.subjectBrain Neoplasmspt_PT
dc.subjectCell Differentiationpt_PT
dc.subjectCell Line, Tumorpt_PT
dc.subjectGliomapt_PT
dc.subjectInflammationpt_PT
dc.subjectProteomept_PT
dc.subjectProteomicspt_PT
dc.titleAdipocyte proteome and secretome influence inflammatory and hormone pathways in gliomapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F80434%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04293%2F2013/PT
oaire.citation.endPage152pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage141pt_PT
oaire.citation.titleMetabolic Brain Diseasept_PT
oaire.citation.volume34pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream5876
person.familyNameCoelho
person.familyNamePrudêncio
person.familyNameFernandes
person.givenNamePedro
person.givenNameCristina
person.givenNameRúben
person.identifier1200571
person.identifier635792
person.identifier.ciencia-idBE18-3DB2-8D79
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.orcid0000-0002-2343-1108
person.identifier.orcid0000-0002-9920-936X
person.identifier.orcid0000-0001-8933-3984
person.identifier.ridAAZ-9239-2020
person.identifier.scopus-author-id57214054449
person.identifier.scopus-author-id6508057930
person.identifier.scopus-author-id57640135700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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