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3-Nitrotyrosine quantification methods: Current concepts and future challenges

2016Journal article Open access
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dc.contributor.authorTeixeira, Dulce
dc.contributor.authorFernandes, Rúben
dc.contributor.authorPrudêncio, Cristina
dc.contributor.authorVieira, Mónica
dc.date.accessioned2017-01-27T12:46:22Z
dc.date.available2017-01-27T12:46:22Z
dc.date.issued2016
dc.description.abstractMeasurement of 3-nitrotyrosine (3-NT) in biological samples can be used as a biomarker of nitrosative stress, since it is very stable and suitable for analysis. Increased 3-NT levels in biological samples have been associated with several physiological and pathological conditions. Different methods have been described for the detection and quantification of this molecule, such as (i) immunological methods; (ii) liquid chromatography, namely high-pressure liquid chromatography (HPLC)-based methods that use ultraviolet-visible (UV/VIS) absorption, electrochemical (ECD) and diode array (DAD) detection, liquid chromatography-mass spectrometry (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS); (iii) gas chromatography, such as gas chromatography-mass spectrometry (GC-MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods A literature review on nitrosative stress, protein nitration, as well as 3-NT quantification methods was carried out. Results This review covers the different methods for analysis of 3-NT that have been developed during the last years as well as the latest advances in this field. Overall, all methods present positive and negative aspects, although it is clear that chromatography-based methods present good sensitivity and specificity. Regarding this, GC-based methods exhibit the highest sensibility in the quantification of 3-NT, although it requires a prior time consuming derivatization step. Conversely, HPLC does not require such derivatization step, despite being not as accurate as GC. Conclusion It becomes clear that all the methods described during this literature review, although accurate for 3-NT quantification, need to be improved regarding both sensitivity and specificity. Moreover, optimization of the protocols that have been described is clearly needed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.biochi.2016.02.011pt_PT
dc.identifier.issn0300-9084
dc.identifier.urihttp://hdl.handle.net/10400.22/9470
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0300908416000547pt_PT
dc.subject3-Nitrotyrosinept_PT
dc.subjectImmunochemical methodpt_PT
dc.subjectNitrosative stresspt_PT
dc.subjectChromatographic methodpt_PT
dc.subjectQuantification methodspt_PT
dc.title3-Nitrotyrosine quantification methods: Current concepts and future challengespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage11pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleBiochimiept_PT
oaire.citation.volume125pt_PT
person.familyNameTeixeira
person.familyNameFernandes
person.familyNamePrudêncio
person.familyNameVieira
person.givenNameDulce
person.givenNameRúben
person.givenNameCristina
person.givenNameMónica
person.identifier635792
person.identifier1200571
person.identifier.ciencia-idF217-0942-4461
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.orcid0000-0002-9388-9018
person.identifier.orcid0000-0001-8933-3984
person.identifier.orcid0000-0002-9920-936X
person.identifier.orcid0000-0002-9614-9031
person.identifier.ridD-8977-2016
person.identifier.scopus-author-id57640135700
person.identifier.scopus-author-id6508057930
person.identifier.scopus-author-id24464652500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc7338403-0b6f-48fb-ac57-30d0e652496d
relation.isAuthorOfPublication9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication55587c4b-3df0-4ff1-bade-587420940ecd
relation.isAuthorOfPublication.latestForDiscovery55587c4b-3df0-4ff1-bade-587420940ecd

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