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Automatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometry

2022Journal article Restricted access
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dc.contributor.authorFernandes, Sara
dc.contributor.authorBarreiros, Luísa
dc.contributor.authorSá, Paula
dc.contributor.authorMiró, Manuel
dc.contributor.authorSegundo, Marcela A.
dc.date.accessioned2021-09-23T11:08:28Z
dc.date.available2021-09-23T11:08:28Z
dc.date.issued2022
dc.description.abstractAn automatic micro-solid-phase extraction (μSPE) method using on-line renewable sorbent beads followed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) was established for the determination of tranexamic acid (TXA) in urine. The μSPE method was based on the bead injection (BI) concept combined with the mesofluidic lab-on-valve (LOV) platform. All steps of the μSPE–BI–LOV were implemented by computer programming, rendering enhanced precision on time and flow events. Several parameters, including the type of sorbent, volume and composition of the conditioning solution, washing solution, and eluent composition, were evaluated to improve the extraction efficiency. The best results were obtained with a hydrophilic–lipophilic balanced mixed-mode sorbent, decorated with sulfonic acid groups (Oasis MCX), and 99% acetonitrile–water (50:50, v/v)–1% ammonium hydroxide as eluent. Chromatographic separation was performed using a BEH amide column coupled to MS/MS detection in positive ionization mode. Good linearity was achieved (R2 > 0.998) for TXA concentrations in urine ranging from 300 to 3000 ng mL−1, with LOD and LOQ of 30 and 65 ng mL−1, respectively. Dilution integrity was observed for dilution factors up to 20,000 times, providing the extension of the upper limit of quantification to 12 mg mL−1. The method was validated according to international guidelines and successfully applied to urine samples collected during scoliosis surgery of pediatric patients treated with TXA.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, S.R., Barreiros, L., Sá, P. et al. Automatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometry. Anal Bioanal Chem 414, 649–659 (2022). https://doi.org/10.1007/s00216-021-03606-ypt_PT
dc.identifier.doi10.1007/s00216-021-03606-ypt_PT
dc.identifier.issn1618-2650
dc.identifier.urihttp://hdl.handle.net/10400.22/18503
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs00216-021-03606-ypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBead injectionpt_PT
dc.subjectLab-on-valvept_PT
dc.subjectAutomationpt_PT
dc.subjectMass spectrometrypt_PT
dc.subjectTranexamic acidpt_PT
dc.subjectUrinept_PT
dc.titleAutomatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometrypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage11pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleAnalytical and Bioanalytical Chemistrypt_PT
person.familyNameFernandes
person.familyNameBarreiros
person.givenNameSara
person.givenNameLuisa
person.identifier.ciencia-id1C12-D800-38A4
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0001-7042-1941
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id57203278917
person.identifier.scopus-author-id6508205485
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication6c2ed5f0-c3fd-4559-99a3-b8d78d019b47
relation.isAuthorOfPublication1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57

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