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Structure and function of a novel antioxidant peptide from the skin of tropical frogs

2018-02Journal article Open access
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dc.contributor.authorBarbosa, Eder Alves
dc.contributor.authorOliveira, Ana
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorSocodato, Renato
dc.contributor.authorPortugal, Camila C.
dc.contributor.authorMafud, Ana Carolina
dc.contributor.authorOmbredane, Alicia S.
dc.contributor.authorMoreira, Daniel C.
dc.contributor.authorVale, Nuno
dc.contributor.authorBessa, Lucinda J.
dc.contributor.authorJoanitti, Graziella A.
dc.contributor.authorAlves, Cláudia
dc.contributor.authorGomes, Paula
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorMascarenhas, Yvonne Primerano
dc.contributor.authorMarani, Mariela M.
dc.contributor.authorRelvas, João B.
dc.contributor.authorPintado, Manuela
dc.contributor.authorLeite, José Roberto S.A.
dc.date.accessioned2020-01-23T15:05:56Z
dc.date.available2020-01-23T15:05:56Z
dc.date.issued2018-02
dc.description.abstractThe amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or UV radiation. Based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. By a peptidomic approach, a novel antioxidant peptide (AOP) with in vitro free radical scavenging ability was isolated from Physalaemus nattereri. The AOP, named antioxidin-I, has a molecular weight [M+H]+ = 1543.69Da and a TWYFITPYIPDK primary amino acid sequence. The gene encoding the antioxidin-I precursor was expressed in the skin tissue of three other Tropical frog species: Phyllomedusa tarsius, P. distincta and Pithecopus rohdei. cDNA sequencing revealed highly homologous regions (signal peptide and acidic region). Mature antioxidin-I has a novel primary sequence with low similarity compared with previously described amphibian's AOPs. Antioxidin-I adopts a random structure even at high concentrations of hydrophobic solvent, it has poor antimicrobial activity and poor performance in free radical scavenging assays in vitro, with the exception of the ORAC assay. However, antioxidin-I presented a low cytotoxicity and suppressed menadione-induced redox imbalance when tested with fibroblast in culture. In addition, it had the capacity to substantially attenuate the hypoxia-induced production of reactive oxygen species when tested in hypoxia exposed living microglial cells, suggesting a potential neuroprotective role for this peptide.pt_PT
dc.description.sponsorshipThe authors thank of the B2Tech platform facilities (Biochemical And Biophysical Technologies, Instituto de Investigação e Inovação em Saúde (I3S) da Universidade do Porto, Portugal) for the use of circular dichroism (CD). AP is grateful for the Fundação para a Ciência e a Tecnologia (FCT, Portugal) grants (SFRH/BD/97995/2013) financed by POPH-QREN (subsidized by FSE and MCTES). NV thanks FCT and FEDER (European Union) for funding through UID/MULTI/04378/2013, project grant IF/00092/2014 and IF2014 position. NV thanks also Fundação Manuel António da Mota (FMAM, Portugal) by support to Nuno Vale Lab. PG thanks FCT for funding through UID/QUI/50006/2013. RS and CCP, hold postdoctoral fellowships from FCT (Refs: SFRH/BPD/91833/2012 and FRH/BPD/91962/2012, respectively). ACM is grateful to FAPESP (Grants 2014/02282-6 and 2016/18023-5). YPM is grateful to CNPq by the Senior Researcher Grant (PQ-Sr 306036/2016-9). EAB is grateful to PNPD/CAPES (Grant No:1603966) for its postdoctoral fellowship. M. M. Marani is a researcher at CONICET.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.freeradbiomed.2017.11.001pt_PT
dc.identifier.issn0891-5849
dc.identifier.urihttp://hdl.handle.net/10400.22/15356
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0891584917311930?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAmphibian Proteinspt_PT
dc.subjectAnimalspt_PT
dc.subjectAntimicrobial Cationic Peptidespt_PT
dc.subjectAntioxidantspt_PT
dc.subjectAnurapt_PT
dc.subjectBacterial Infectionspt_PT
dc.subjectCloning, Molecularpt_PT
dc.subjectFibroblastspt_PT
dc.subjectFree Radical Scavengerspt_PT
dc.subjectMicept_PT
dc.subjectMicrogliapt_PT
dc.subjectMolecular Structurept_PT
dc.subjectNIH 3T3 Cellspt_PT
dc.subjectNeuroprotectionpt_PT
dc.subjectOxidation-Reductionpt_PT
dc.subjectProtein Conformationpt_PT
dc.subjectReactive Oxygen Speciespt_PT
dc.subjectSkinpt_PT
dc.titleStructure and function of a novel antioxidant peptide from the skin of tropical frogspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97995%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F91833%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F91962%2F2012/PT
oaire.citation.endPage79pt_PT
oaire.citation.startPage68pt_PT
oaire.citation.titleFree Radical Biology and Medicinept_PT
oaire.citation.volume115pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream5876
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
person.familyNamePlácido
person.familyNameDelerue-Matos
person.givenNameAlexandra
person.givenNameCristina
person.identifier1621053
person.identifier.ciencia-id1B1A-461B-E8DD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0003-2706-7777
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridL-3085-2014
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id55232677900
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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