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Advisor(s)
Abstract(s)
Na última década têm desenvolvido fixadores para substituição do formol que é tóxico para o homem. O principal objectivo foi avaliar microscopicamente a histomorfologia e as características tintoriais de tecido mamário fixado em GreenFix®, durante 24 e 72 horas, comparativamente ao fixado em formol, através da coloração de Hematoxilina-Eosina. Uma análise global da histomorfologia revelou existir uma diferença estatisticamente significativa entre a fixação pelo GreenFix® e pelo formol (p=0,050), tendo-se registado uma melhoria da coloração e detalhe nuclear nos tecidos fixados com GreenFix® durante 24 (p=0,007) ou 72 horas(p=0,024). O GreenFix® é um potencial substituto do formol na rotina histológica.
In the last decade fixatives to substitute the formaldehyde - that is toxic for man – have been developed. The main goal was to evaluate the morphology and histological dyeing features of breast tissue fixed by GreenFix® during 24 and 72 hours comparing to those fixed by formaldehyde, through hematoxylin-Eosin staining. A global analysis of histomorphology showed statistically significant difference between GreenFix® and formaldehyde fixation (p=0,050). An improvement on nuclear detail and staining was achieved by GreenFix® fixation during 24 (p=0,007) or 72 hours (p=0,024). The GreenFix® is a potential substitute of formaldehyde for histological routine.
In the last decade fixatives to substitute the formaldehyde - that is toxic for man – have been developed. The main goal was to evaluate the morphology and histological dyeing features of breast tissue fixed by GreenFix® during 24 and 72 hours comparing to those fixed by formaldehyde, through hematoxylin-Eosin staining. A global analysis of histomorphology showed statistically significant difference between GreenFix® and formaldehyde fixation (p=0,050). An improvement on nuclear detail and staining was achieved by GreenFix® fixation during 24 (p=0,007) or 72 hours (p=0,024). The GreenFix® is a potential substitute of formaldehyde for histological routine.
Description
Keywords
Tecido mamário Morfologia Formol GreenFix® Breast tissue Morphology Formaldehyde
Citation
Publisher
Instituto Politécnico do Porto. Escola Superior de Tecnologia da Saúde do Porto - Politema