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Advisor(s)
Abstract(s)
The use of 3D cell culture for modelling cancer research is gaining interest, and in particular for screening of bioactive compounds. In the presented work, three compounds isolated from cyanobacteria and 92 chromatographic fractions obtained from crude organic extracts of 28 cyanobacterial strains from the Portuguese coast were tested on multicellular spheroids generated from the human cell lines HCT116 (colon colorectal carcinoma), HT-29 (colon adenocarcinoma) and A549 (lung carcinoma). Spheroids were incubated and analysed in an IncuCyte® ZOOM System, and M30 Cytodeath ELISA was performed for apoptosis. Portoamides isolated from Oscillatoria sp. LEGE 05292 reduced HCT116 spheroid integrity. Bartoloside B from Synechocystis salina LEGE 06155 decreased the size of HCT116 spheroids. Compound A isolated from Nodularia sp. LEGE 06071 induced a loss of the boundaries of the spheroid. Beyond that, portoamides induced apoptosis on HCT116 and HT29 spheroids, and compound A on HCT116 and A549 spheroids. The screening revealed that five fractions reduced the spheroid integrity; isolation of the active components will be done in the future by bioassay-guided fractionation. We conclude that the tested compounds and extracts show biological activities on multicellular spheroids and are therefore interesting candidates to be further developed as therapeutics for solid tumors.
Description
Keywords
3D cell culture cyanobacteria
Citation
Sousa, M. L., Preto, M., Leão, P., Martins, R., Linder, S., Vasconcelos, V., & Urbatzka, R. (2017, março). Generation of spheroids of cancer cells for screening of antitumour activity of cyanobacterial extracts and isolated compounds. BIOPROSP, the 8th International Conference on Marine Biotechnology.
Publisher
CIIMAR