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Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli

2016Journal article Restricted access
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dc.contributor.authorMarani, Mariela M.
dc.contributor.authorPerez, Luis O.
dc.contributor.authorAraujo, Alyne Rodrigues de
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorSousa, Carla F.
dc.contributor.authorQuelemes, Patrick Veras
dc.contributor.authorOliveira, Mayara
dc.contributor.authorGomes-Alves, Ana G.
dc.contributor.authorPueta, Mariana
dc.contributor.authorGameiro, Paula
dc.contributor.authorTomás, Ana M.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorEaton, Peter
dc.contributor.authorCamperi, Silvia A.
dc.contributor.authorBasso, Néstor G.
dc.contributor.authorLeite, Jose Roberto de Souza de Almeida
dc.date.accessioned2017-01-26T11:29:32Z
dc.date.embargo2117
dc.date.issued2016
dc.description.abstractPatagonia's biodiversity has been explored frommany points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 μM). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttp://dx.doi.org/10.1016/j.gene.2016.12.020pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/9427
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesGene;Vol. 605
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0378111916310204pt_PT
dc.subjectAnurapt_PT
dc.subjectAtomic force microscopypt_PT
dc.subjectcDNApt_PT
dc.subjectCircular dichroismpt_PT
dc.subjectSurface Plasmon Resonancept_PT
dc.titleThaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia colipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage80pt_PT
oaire.citation.startPage70pt_PT
oaire.citation.titleGenept_PT
oaire.citation.volume605pt_PT
person.familyNameDelerue-Matos
person.givenNameCristina
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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