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Impedimetric immunosensors for the detection of Cry1Ab proteinfrom genetically modified maize seeds

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dc.contributor.authorFreitas, Maria
dc.contributor.authorCorrer, Wagner
dc.contributor.authorCancino-Bernardi, Barroso
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorZucolotto, Valtencir
dc.date.accessioned2017-01-12T12:54:54Z
dc.date.embargo2116
dc.date.issued2016
dc.description.abstractRegardless the controversies surrounding genetically modified organisms (GMO), their cultivation isconstantly increasing and in according to the EU legislation, labeling is mandatory for products con-taining EU-authorized-GMO higher than 0.9%. Thereby, new analytical strategies for rapid and effectivedetection of GMO on foodstuffs are required. In this work, an electrochemical immunosensor foreffective determination of Cry1Ab protein from MON810 transgenic maize (EU-authorized-GMO) isdescribed. The immunosensor was developed onto indium tin oxide (ITO) electrodes modified by3-aminopropyltrimethoxysilane (APTES) monolayer to covalently immobilize Anti-Cry1Ab polyclonalantibodies. The protein interaction with the polyclonal antibody (PAb) recognition platform was directlymonitored and measured by cyclic voltammetry and electrochemical impedance spectroscopy usingcommercially Cry1Ab protein. After the analytical features optimization a linear response from 1 to10 ng mL−1, a limit of detection (LOD) of 0.37 ng mL−1and a limit of quantification (LOQ) of 1.23 ng mL−1– which provided accurate results (RSD < 7.5%) – were achieved. The immunosensor allowed a simple andfast detection of Cry1Ab protein extracted from maize seeds with different GM maize mass percentages(0%, 0.5%, 1%, 2.5% and 5%). To crosscheck the detection of Cry1Ab protein, an enzyme-linked immunosor-bent assay (ELISA) was used. The results indicate that the immunosensor is suitable for the transgenicprotein Cry1Ab detection in GM maize representing a successfully tool to verify the compliance of theEU regulations.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2016.06.149pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/9250
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesSensors and Actuators B: Chemical;Vol. 237
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0925400516310115pt_PT
dc.subjectElectrochemical immunosensorpt_PT
dc.subjectCry1Abpt_PT
dc.subjectElectrochemical impedance spectroscopypt_PT
dc.subjectGenetically modified maizept_PT
dc.titleImpedimetric immunosensors for the detection of Cry1Ab proteinfrom genetically modified maize seedspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage709pt_PT
oaire.citation.startPage702pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume237pt_PT
person.familyNameDelerue-Matos
person.givenNameCristina
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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