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Complex osteoclastogenic inductive effects of nicotine over hydroxyapatite

2018Journal article Open access
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dc.contributor.authorCosta-Rodrigues, João
dc.contributor.authorRocha, Isabel
dc.contributor.authorFernandes, Maria H.
dc.date.accessioned2019-07-01T11:42:48Z
dc.date.available2020-05-02T00:30:30Z
dc.date.issued2018
dc.description.abstractCigarette smoke is associated to pathological weakening of bone tissue, being considered an important playmaker in conditions such as osteoporosis and periodontal bone loss. In addition, it is also associated with an increased risk of failure in bone regeneration strategies. The present work aimed to characterize the effects of nicotine on human osteoclastogenesis over a hydroxyapatite substrate. Osteoclast precursors were maintained in the absence or presence of the osteoclastogenesis enhancers M-CSF and RANKL, and were further treated with nicotine levels representative of the concentrations observed in the plasma and saliva of smokers. It was observed that nicotine at low concentrations elicit an increase in osteoclast differentiation, but only in the presence of M-CSF and RANKL it was also able to significantly increase the resorbing ability of osteoclasts. A slight downregulation of NFkB pathway and an increase in the production of TNF-α and, particularly PGE2, were involved in the observed effects of nicotine. At high concentrations, nicotine revealed cytotoxic effects, causing a decrease in cell density. In conclusion, nicotine at levels found in the plasma of the smokers, has the ability to act directly on osteoclast precursors, inducing its osteoclastogenic differentiation. The stimulatory behavior appears to be dependent on the stage of osteoclastic differentiation of the precursor cells, which means, in the absence of M-CSF and RANKL, it only favors the initial stages of osteoclast differentiation, while in the presence of the growth factors, a significant increase in their resorbing ability is also achieved.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta-Rodrigues, J., Rocha, I., & Fernandes, M. H. (2018). Complex osteoclastogenic inductive effects of nicotine over hydroxyapatite. Journal of Cellular Physiology, 233(2), 1029–1040. https://doi.org/10.1002/jcp.25956
dc.identifier.doi10.1002/jcp.25956pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14176
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationPOCI/01/0145/FEDER/007265pt_PT
dc.relationPT2020 UID/QUI/50006/2013pt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/jcp.25956pt_PT
dc.subjectHydroxyapatitept_PT
dc.subjectNicotinept_PT
dc.subjectCell Differentiationpt_PT
dc.subjectOsteoclastogenesispt_PT
dc.subjectDinoprostonept_PT
dc.subjectDurapatitept_PT
dc.subjectNF-kappa Bpt_PT
dc.subjectOsteoclastspt_PT
dc.subjectOsteogenesispt_PT
dc.subjectRANK Ligandpt_PT
dc.subjectSignal Transductionpt_PT
dc.subjectTartrate-Resistant Acid Phosphatasept_PT
dc.subjectTime Factorspt_PT
dc.subjectTumor Necrosis Factor-alphapt_PT
dc.titleComplex osteoclastogenic inductive effects of nicotine over hydroxyapatitept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1040pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage1029pt_PT
oaire.citation.titleJournal of Cellular Physiologypt_PT
oaire.citation.volume233pt_PT
person.familyNameCosta-Rodrigues
person.givenNameJoao
person.identifier.ciencia-id7412-B006-2120
person.identifier.orcid0000-0003-1375-8067
person.identifier.scopus-author-id8558383400
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationce85cc8f-09f3-4c26-aeaf-6128971acf68
relation.isAuthorOfPublication.latestForDiscoveryce85cc8f-09f3-4c26-aeaf-6128971acf68

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