Repository logo
 
Publication

Celiac disease detection using a transglutaminase electrochemical immunosensor fabricated on nanohybrid screen-printed carbon electrodes

2012Journal article Restricted access
Simple item page
dc.contributor.authorNeves, Marta M. P. S.
dc.contributor.authorGonzález-García, María Begoña
dc.contributor.authorNouws, Henri P. A.
dc.contributor.authorCosta-García, Agustín
dc.date.accessioned2013-09-27T10:31:59Z
dc.date.available2013-09-27T10:31:59Z
dc.date.issued2012
dc.description.abstractCeliac disease is a gluten-induced autoimmune enteropathy characterized by the presence of tissue tranglutaminase (tTG) autoantibodies. A disposable electrochemical immunosensor (EI) for the detection of IgA and IgG type anti-tTG autoantibodies in real patient’s samples is presented. Screen-printed carbon electrodes (SPCE) nanostructurized with carbon nanotubes and gold nanoparticles were used as the transducer surface. This transducer exhibits the excellent characteristics of carbon–metal nanoparticle hybrid conjugation and led to the amplification of the immunological interaction. The immunosensing strategy consisted of the immobilization of tTG on the nanostructured electrode surface followed by the electrochemical detection of the autoantibodies present in the samples using an alkaline phosphatase (AP) labelled anti-human IgA or IgG antibody. The analytical signal was based on the anodic redissolution of enzymatically generated silver by cyclic voltammetry. The results obtained were corroborated with a commercial ELISA kit indicating that the electrochemical immunosensor is a trustful analytical screening tool.por
dc.identifier.doi10.1016/j.bios.2011.09.044pt_PT
dc.identifier.issn0956-5663
dc.identifier.urihttp://hdl.handle.net/10400.22/2013
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofseriesBiosensors and Bioelectronics; Vol. 31, Issue 1
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0956566311006658por
dc.subjectTransglutaminasepor
dc.subjectCeliac diseasepor
dc.subjectScreen-printed electrodespor
dc.subjectCarbon nanotubespor
dc.subjectGold nanoparticlespor
dc.titleCeliac disease detection using a transglutaminase electrochemical immunosensor fabricated on nanohybrid screen-printed carbon electrodespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage100por
oaire.citation.issueIssue 1por
oaire.citation.startPage95por
oaire.citation.titleBiosensors and Bioelectronics
oaire.citation.volumeVol. 31por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
ART_MNeves_2012_GRAQ_SupplMater.pdf
Size:
228.61 KB
Format:
Adobe Portable Document Format
Description:
Supplementary material
Download
No Thumbnail Available
Name:
ART_MNeves_2012_GRAQ.pdf
Size:
653.94 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEP – GRAQ – Artigos